Papillomavirus Part 4: Equine Canker

From Guest Author Dr. Angelika Schoster, Department of Clinical Studies, University of Guelph.

Equine hoof canker (pododermatitis chronica verrucosa) is described as a chronic, proliferative dermatitis, beginning in the caudal part of the cleft of the frog and gradually expanding to the sole and wall. Equine canker is not lethal in and of itself, but because of where it occurs on the foot, and because it can be so difficult to treat and it recurs so often, it can severely compromise a horse’s ability to do its job (even if that job is just walking around in a field sometimes). The etiology of equine canker has been a topic of discussion for over 50 years, but so far no specific cause for the disease has been found.

Canker is usually diagnosed based on the appearance of the affected horny tissue and a distinctly fetid odour. The definitive diagnosis has to be confirmed by microscopic examination (histopathology) of biopsies collected from the affected tissue.

Treatment of canker has always been a dilemma for veterinarians and farriers because it is so difficult. Treatments used today can range from surgical intervention (removal of the affected tissue) to conservative medical management using topical medications of many different kinds.

The proliferative but non-metastatic nature of canker is similar to that of equine sarcoids. Like canker, sarcoids also tend to be difficult to treat and often recur. Both canker and sarcoids often include a mixture of proliferative and erosive changes in the skin secondary to overgrowth and thickening of the tissues. Due to these similarities, it has been speculated that bovine papillomavirus (BPV) might also be involved in causing canker. A large study in Europe (which will hopefully be published soon) has found evidence to suggest that a variant of BPV-1 plays a role in the development and/or maintenance of canker lesions in horses. Much more study is still needed, but this is another interesting example of how an infectious pathogen may ultimately play a role in a disease that’s origin has remained a mystery for decades.

More information about papillomaviruses is available in our archives.

Image: Severe canker in the frog of a horse's hoof (source: www.answers.com)

Papillomavirus Part 3: Sarcoid Stresses

From Guest Author Dr. Angelika Schoster, Department of Clinical Studies, University of Guelph.

Papillomaviruses are generally strictly species-specific, meaning a certain type of virus will only infect one animal species. Infection of horses with bovine papillomavirus is the only known example (so far) of cross-species infection within this group of viruses. In cattle, bovine papillomaviruses (BPVs) cause warts, just like equine papillomaviruses cause warts in horses. It is now widely accepted that bovine papillomaviruses (BPV-1 and BPV-2) are also the primary cause of equine sarcoids. More recently, these viruses have also been implicated in some cases of equine dermatitis and equine hoof canker (more on that in another post).

Equine sarcoids are skin tumours that affect horses, donkeys, mules and zebras. They are the most common type of tumour found in horses, and account for 35-90% of all equine skin tumours. Sarcoids are generally not life-threatening – unlike some other tumours, they do not metastasize (i.e. spread to other organs or tissues). However, they can be locally aggressive growths, meaning they may invade deep into the tissues immediately surrounding the primary tumour. They are also very difficult to treat, and therefore can affect a horse’s welfare or quality of life, depending on the size and location of the sarcoid. There is some evidence of a genetic predisposition to sarcoids in some horses as well. Involvement of bovine papillomavirus in the development of sarcoids was first suggested by Olson and Cook in 1951, who were able to reproduce sarcoid-like lesions in horses by inoculating them with tissue from bovine warts. Since then, researchers have used PCR to show that the vast majority of sarcoids contain BPV DNA, whereas normal horses and non-sarcoid skin tumours do not. Although BPV DNA has been found in healthy horses living in close contact with sarcoid-bearing animals, there is currently no evidence that BPV or sarcoids are transmissible horse-to-horse.

Six types of equine sarcoid are described based on their appearance: occult, verrucous, nodular, fibroblastic, mixed or malevolent. Each type tends to occur on different parts of the body, and they also differ in how they behave (i.e. how fast they grow). However, all six types share the tendency to recur and become more aggressive if they are disturbed accidentally (e.g. traumatized) or iatrogenically (e.g. surgically (but incompletely) removed). Unlike warts, sarcoids rarely disappear on their own (resolve spontaneously). Many different techniques have been used to try to treat sarcoids, including surgical removal, cryotherapy (freezing), carbon dioxide laser therapy, hyperthermia (burning), irradiation, photodynamic therapy, immunotherapy, and chemotherapy. Unfortunatley, to date there is no universally effective treatment for sarcoids (which is why so many different things have been tried and continue to be tried).

There is currently no effective vaccine against sarcoids, but knowing that a virus (BPV) is involved may ultimately allow researchers to one day develop a vaccine or other means of prevention. In the meantime, it probably wouldn’t hurt to keep your horse away from any warty cows that may be around.

Photo: A horse with recurrent aggressive sarcoids on the medial aspects of both forelegs (credit: M. Anderson)

Papillomavirus Part 2: Plaque Problems

From Guest Author Dr. Angelika Schoster, Department of Clinical Studies, University of Guelph.

No, not dental plaque like you get on your teeth - in this case we’re talking about aural plaques, which are a type of skin lesion that some horses develop on the inside surface of their ears. These plaques are usually depigmented, meaning the skin cells of which they are made do not have the same pigment in them as the cells of the surrounding skin, making the lesions appear pale grey or off-white. Other than being considered unsightly in some cases, aural plaques usually don’t cause problems for the horse – they’re not itchy, sensitive or painful. They can occur in any horse, no matter the age, breed or sex. Occasionally some horses may develop similar plaques on the udder or around the anus or vulva (under the tail). The diagnosis of an aural plaque is typically made based on the horse’s clinical signs alone (i.e. a raised, depigmented skin lesion inside the ear that is not associated with any inflammation or discomfort).

The exact cause of aural plaques is still not clear, but using extremely high-powered electron microscopy, researchers have been able to demonstrate that in some cases there is a papillomavirus infecting the cells within these plaques. No one has yet been able to isolate the virus to determine what type of papillomavirus it might be. Aural plaques are not known to be transmissible from horse to horse, but since there may be a virus involved, it's prudent to consider the possibility that virus transmission may play a role in their development (although there are likely many other factors involved as well).  Unlike regular papillomas (i.e. warts, caused by equine papillomaviurs), aural plaques do not disappear on their own. It the past, the only way to remove the plaques was to actually cut them away surgically. In more recent years, successful treatment has been reported using an immune-response modifier cream such as imiquimod (Aldara), which is used in people to treat several different skin conditions, including genital warts.

Photo: Aural plaque on the ear of a horse (source: University of Minnesota - College of Veterinary Medicine)

Papillomavirus Part 1: Wart Worries

From Guest Author Dr. Angelika Schoster, Department of Clinical Studies, University of Guelph.

Papillomaviruses (PVs) are small DNA-based viruses that are recognized causes of disease in many animal species and humans. More than 100 different human papillomavirus types (HPV) have been identified, which can cause a range of problems from annoying (but benign) warts to malignant cervical cancer. The ability of papillomaviruses to cause cancer was first recognized in animals, specifically with cottontail rabbit papillomavirus (CRPV), bovine papillomavirus (BPV) and canine oral papillomavirus (COPV). In general, PV infection causes benign disease in the vast majority of individuals, but the viruses have the potential to cause malignant disease in a small proportion of the mammals they infect as well.

Horses can be infected by both equine papillomavirus (EPV) and some bovine papillomaviruses. Infection with BPV is associated with equine sarcoids – look for more information on BPV in horses in an upcoming post. Infection with EPV (specifically Equuus caballus papillomavirus types I and II) causes cutaneous papillomas – small proliferative skin lesions better known as warts. Warts are benign growths that appear on animals less than two years of age. They typically persist for 6-12 months and then disappear without any specific treatment, and they do not otherwise make the animal sick. Usually warts appear on the muzzle and lips, but rarely genital warts can occur (usually with infection by the type II virus). Occasionally warts can also occur on the lower limbs, ears and eyelids.

Warts can be spread between horses by fomites (e.g. objects, equipment, tack, clothing that has been contaminated with EPV), or by close horse-to-horse contact. Spread is common when young are horses are brought together in large groups for shows, sales or breeding. Infection is usually diagnosed by the characteristic skin lesions, the horse’s history and age - more than 90% of affected horses are less than three years old. Usually the warts regress spontaneously and they do not require treatment. However, if treatment is sought for esthetic reasons, cryosurgery (i.e. freezing, usually with something like liquid nitrogen) can be performed. Caustic chemicals such as trifluoracetic acid can also be used to “burn” the warts off. Even after treatment, though, the warts can recur.

Controlling warts caused by EPV is fairly simple: if you have an affected horse, keep it away from other young horses. Do not allow the horses to have direct contact, and make sure the horse with the warts has its own tack, grooming supplies, water bucket, hay net/trough etc. so the virus is not indirectly transmitted to others. There is no vaccine available for horses for EPV. There are a lot of “voodo” treatments for warts out there, and some people swear by them, but (just like people who treat foal heat diarrhea) that’s usually because they’re treating a condition which goes away on its own anyway. People cannot be infected by EPV.

Photo: Warts on the nose and lips of a horse (source: http://www.yourveterinaryclinic.com)