Rabid Horse In Texas

A rabid horse has been identified in Bandera County, Texas - this is the first case of rabies in a Texas horse in almost 40 years. The horse started acting strangely, erratically and at times violently. Rabies was eventually diagnosed (diagnosis involves testing the brain after the horse dies or is euthanized) and the owners are undergoing post-exposure treatment.

Rabies is a rare disease in horses, but there is always a risk of exposure in regions where rabies is present in wildlife. The fact that equine rabies is rare is a good thing, but it can also breed complacency and lead to decreased vaccination rates.

Decreasing vaccination is a bad thing. Vaccination is cheap insurance for a very serious disease. Rabies is invariably fatal in horses. It's also a risk to anyone around the horse. Transmission of rabies from horses to people is theoretically possible through bites or other contacts that result in introduction of saliva into wounds, cuts or possibly the mouth, nose or eyes. I'm not aware of any documented cases of equine-to-human transmission of rabies, but given the severity of rabies, we need to be careful. Additionally, rabid horses have killed people because they can be unpredictable and aggressive. Based on all these factors, it's important that we not become complacent about rabies in horses, and a key component of rabies control is vaccination. However, vaccination is not the only aspect of rabies control. Using various management practices to reduce the risk of wildlife exposure and controlling rabies in wildlife in the area are other important measures, but individual horse owners can have the greatest impact on their horses' health by ensuring they are properly vaccinated against rabies.

If you own a horse, make sure it's vaccinated against rabies unless you live in a rabies-free region.  More information about rabies in horses is available on the equIDblog Resources page.

Image source: www.bbc.co.uk

Alcohol Wipe Before Injection?

It's common for people to wipe injection sites in horses with alcohol before inserting the needle. It's so ingrained into some peoples' minds that they may complain if their veterinarian doesn't use an alcohol wipe before injecting. But what does that little swab really do, and is it needed?

Alcohol is a disinfectant and can kill many (but not all) bacteria and viruses. Therefore, wiping an injection site with alcohol could theoretically reduce bacterial counts and maybe reduce the risk of an injection site infection. However, alcohol does not work well in the presence of organic debris (i.e. dirt), and may not (and likely won't in most cases) penetrate through a horse's haircoat down to the skin. The best aspect of alcohol is some situations is wetting down the hair to help see the area you're injecting (for example, when visualizing the vein for an intravenous injection).

The risk of injection site infection is very low in the average horse, and the true benefit of wiping the site with alcohol is unknown. There is no clear evidence that this practice reduces infections. The most serious injection-associated infection, clostridial myonecrosis, is caused by either inoculation of clostridial spores (which are resistant to alcohol) or by spores that are dormant in the muscle tissue. In either case, wiping the skin with alcohol won't help prevent it.

Bottom line: There's no evidence that alcohol wiping is needed, or useful. There's also no reason not to do it, so it's really a matter of personal preference. Not using alcohol wipes is not an indication of poor practice, but a lot of people will use them on the chance that they are effective, knowing that at worst they will do no harm.

More information about clostridial myonecrosis can be found on the equIDblog Resources page.

Helicobacter Testing For Horses

I've had a run of questions about testing for Helicobacter in horses with gastric (i.e. stomach) ulcers. Helicobacter pylori is unquestionably an important problem and clear cause of gastric ulcers in people. However, people aren't horses. The human stomach differs greatly from the equine stomach, and gastric ulcers in people are much different than gastric ulcers in horses. There is no (I repeat, NO) evidence that Helicobacter is a cause of ulcers in horses. Despite this some unscrupulous molecular diagnostic companies offer unvalidated tests for Helicobacter in horses, for reasons that make absolutely no sense.

Don't waste your money on Helicobacter testing if your horse has ulcers. Spend it on treatment and management changes to reduce the risks of ulcers recurring.

(click image for source)

Review Of CEM and Implications For Canada

Hard to believe that the whole conundrum about contagious equine metritis (CEM) that is still affecting the Canadian equine industry started a little bit more than a year ago, when a Quarter Horse stud in Kentucky tested positive for the causative agent, Taylorella equigenitalis. Tracing the contacts of that stud lead to the quarantine and testing of 990 horses in 40 states, as well as 19 mares in Canada, including 9 in Ontario. Out of 274 exposed stallions, 22 were ultimately found to be positive, none of which had any detectable clinical signs that they were infected, meaning if they hadn’t been tested, they may have kept on going about their business breeding – and possibly infecting – mares for years to come. The rest of the exposed horses were mares, five of which turned out to be infected. One particularly interesting fact was that four of these mares had been bred by artificial insemination - previous to this it was thought that CEM was only readily transmissible by live cover from an infected stud, not via semen shipped in a straw.

Thankfully all of the Canadian mares were negative, but unfortunately the Canadian equine industry is still suffering the consequences of what has happened to our close southern neighbour. Fourteen countries have increased import requirements for Canadian (and obviously US) horses in terms of CEM testing, and another major blow was the loss of Canada’s low-risk status with the UK's Horserace Betting Levy Board (HBLB).

Canada must maintain strict import requirements for horses to prevent CEM from getting into the country. Most of the horses imported into Canada come from CEM-positive countries, and it’s getting more difficult to argue not including the US on that list as well. The risk is constantly present, and remaining diligent about quarantine and testing – and rules like ensuring horses are not on antibiotics for some other reason when they’re cultured – is key. Semen import restrictions for semen coming from the US to Canada will stand for 2010. While this certainly causes a headache for breeders, requiring an import permit and a health certificate for the stud stating that it has not stood on a CEM-quarantined farm, is it enough to protect Canadian horses? The next step would be to require all studs to be tested for CEM before their semen can be imported to Canada. That is no small request. Testing a stud involves culturing the stud himself, and then having him test-breed two mares which then also need to be culture-negative. Anecdotally the entire process can cost in the neighbourhood of $5-7K per horse, which at the moment all needs to be borne by the horse owner. That gives you some idea of what an enormous undertaking it was to quarantine and test 274 exposed stallions during last year’s outbreak.

In the US, 87% of exposed horses have now been cleared, and there have been no new positives in the last 8 months. However, there are still 17 states where there are quarantined farms. There is talk of voluntary testing of over 2000 studs in the US in 2010, as well as targeted surveillance of stallions imported in the last 10 years and those standing at large AI centers. Only time will tell if these extra efforts will serve to calm the fears of countries that are now hesitant to import horses and semen from the US, or whether they will reveal more cases of CEM and confirm the fears that CEM may have unknowingly become endemic in the US in the past decade.

More information on CEM is available in our archives.

This blog is based on a presentation by Dr. Tracey Chenier, a theriogenologist and faculty member at the Ontario Veterinary Collge, given at the recent 2010 Ontario Veterinary Medical Association Conference.

Photo credit: David Campbell (click for source)

2008 Australian Hendra Virus Recap

The latest edition of the journal Emerging Infectious Diseases contains a paper describing the 2008  Australian Hendra virus outbreak in horses and people.

In this outbreak, there were five horses infected and two humans infected. The horses predominantly had signs of neurological disease, not respiratory disease like some other reports describing this disease. Four horses died. One recovered but was euthanized for public health reasons.

Two people became infected after working with the sick horses, which represents 10% of the total veterinary staff that were exposed to the infected horses.  Both people started off with influenza-like illness, which seemed to improve initially, but then signs of severe neurological disease developed. They were treated with ribavirin, an antiviral drug, as part of an experimental treatment. One of them died after 40 days of illness, the other person survived.

The authors stressed that the effectiveness of ribavirin could not be determined, but they recommend it nonetheless because of the severity of Hendra virus infection and lack of other options. Ribavirin was also used in the 2009 outbreak, but it is clearly not 100% effective since one person died there also.

A number of concerning activities occurred that put people at risk of infection, including a "percutaneous blood exposure while euthanizing an infected horses" (they didn't explain exactly what this was, but it could have been a needlestick), low use of personal protective equipment, and contact with potentially infectious body fluids. This is unfortunately not surprising since the approach to infection control (particularly in terms of zoonotic infections) is often lax in equine medicine. That certainly has to change, particularly in areas where Hendra virus may be present.

Much more information about how to control this potentially devastating virus is needed. Fortunately, infections are uncommon and it is restricted to a fairly small geographic range in Queensland, Australia.

Image source: http://animalphotos.info/

Bare Feet And Horse Bugs

I assume that people wouldn't voluntarily and regularly walk around barefoot on dog feces (or feces of any type), yet it's perplexing that some people regularly clean out horse stalls in bare feet (I've seen it done!). While horse manure may not be as inherently gross as dog poop, it's still feces, and like all feces contains a huge population of various bacteria, some of which can be harmful. The risks of barefoot mucking may also extend to bare feet inside boots, although I don't think sock-averse people need to panic.

An article in the International Journal of Infectious Diseases (Friederichs et al) describes infectious arthritis of the shoulder of a horse owner that was caused by Streptococcus zooepidemicus, a bacterium commonly found in horses but rarely associated with disease in people. The person didn't have a wound in the shoulder area or any other obvious route for the bacterium to get to the shoulder joint. They searched for a source of the infection and all they found was a chronic lesion on the person's foot. This, combined with the patient's history of taking care of his horses in "bare feet in boots", led them to implicate the foot as the source of infection.

The idea, I guess, is that socks would be a barrier to help prevent contamination of the foot wound. That makes sense to a degree - the person could contaminate his foot with S. zooepidemicus from his hands (probably acquired from touching the horse's nose) while removing the boots, or manure could work its way into boots and directly contaminate the wound. Both are possible, but we have to be a little cautious in interpreting these conclusions. However, this is a bacterium that is associated with horses and the foot lesion is certainly a possible route of entry.

Overall, this should be considered an interesting report of a very rare problem, not something that indicates a major concern. However, there are a few good points to take away from this story:

  • If you have a wound or chronic lesion of any sort, make sure you take measures to reduce the risk of bacterial contamination when working around horses. This might be as simple as making sure it's covered by clothing, or something more involved like using an impermeable bandage.
  • Hands are probably the major source of infection transmission, and good hand hygiene is important after horse or stall contact, particularly if you have an underlying disease.

This equIDblog entry was originally posted on our sister site, Worms & Germs Blog, on 20-Jan-10.

How To Approach Rabies Exposure In Horses

I received newsletter today from Intervet (a pharmaceutical company) that is targeted at equine veterinarians. One article discussed rabies in horses. It wasn't bad overall, but I thought the section on what to do when a horse might have been exposed to rabies was worth discussing.

The article asks, "If your client suspects that a horse has been bitten by a rabies-infected animal, what should be done?"

Answer: "Contacting you as the veterinarian is always the first step."

Great first step.  A second step that wasn't mentioned should be, "Try to identify and (safely) capture the animal that bit the horse." This is often impossible but certainly worthwhile if it can be done.  However, if you're trying to catch the offending animal, make sure you don't put yourself at risk of exposure to rabies in the process.  If the animal can be caught, it's rabies status at the time of the bite can be determined (either through testing or quarantine). If it can be shown that the animal wasn't rabid, a lot of stress, hassle and expense can be saved.

"If the horse was previously vaccinated... Then isolate and observe the animal for 45 to 90 days (your clinical evaluation will involve gait analysis, radiography and a spinal tap)."

Boosting the rabies vaccine is also a good idea. The next step, however, needs to be contacting local regulatory officials to find out what you have to do. They determine if, how and how long an animal needs to be quarantined - this is NOT the decision of the local veterinarian nor the animal's owner. Most likely, they will recommend a 45 day quarantine for a vaccinated horse, since this is what is recommended in the NASPHV Compendium on Rabies. The discussion of diagnostic testing makes no sense. There is absolutely no indication to perform diagnostic tests on a horse that has been bitten by a rabies suspect. None. There are no tests that can be used to diagnose rabies in live horses (also exposed horses don't instantly develop signs of rabies). Horses should be monitored closely for signs of rabies during the quarantine period, but that's it.

"...and have the client make a list of all people who had contact with the horse."

This is often done when horses have or are suspected of having rabies, but not horses that are potentially exposed. It is done to help public health personnel contact people that may have been exposed to rabies. A horse that was just bitten by an animal is not a risk for transmission of rabies.  (However, keeping a list of people who have contact with the horse after it's been bitten (i.e. durng the quarantine period) - which should be as short a list as possible - is a reasonable precaution in the unlikely event that the horse does develop rabies.)

"If the animal was not vaccinated, your options are to euthanize and perform a postmortem examination of the brain (the only way to definitely confirm rabies)..."

Euthanasia is one of the options that needs to be considered in an unvaccinated horse that has been exposed, which is one of the reasons that identifying the biting animal and testing it is critical, if it can be done. The last part of the above sentence (from the atricle) is complete nonsense. Why would you test the brain of a normal horse that has been euthanized because it's just been bitten by a potentially rabid animal? The horse isn't being euthanized because it has rabies, it's being euthanized because of the likelihood  of it developing rabies weeks to months later. Testing of the brain will tell you absolutely nothing if the animal was only bitten recently.

"...or isolate and observe the horse for six months and develop the human contact list."

Again, this needs to be decided based on discussions with regulatory personnel who are responsible for dictating what is to be done. A six-month quarantine is a pretty standard recommendation for an unvaccinated animal. Creating a human contact list should not be necessary, since quarantine involves severely restricting contact of people with the horse and only a few (ideally one) person would have any type of contact.

The article wraps up with the very true emphasis on vaccinating horses. It's a cheap measure to prevent a relatively rare but invariably fatal disease.

Click image for source.

Piroplasmosis In New Mexico

At some point, the US is going to have to admit that piroplasmosis, the bloodborne parasitic infection caused by Theileria equi, is endemic in some regions of the country. It's a declaration that will have major impacts on horse movement to some areas but, it's better for everyone to know what's going on. Piroplasmosis is technically still considered an exotic disease in the US, but there have been many cases identified over the past year and a clear source for the individual outbreaks in lacking, indicating there must be a reservoir in some part(s) of the country.

The latest incident involves the diagnosis of piroplasmosis in three race horses in New Mexico, which were picked up as part of routine screening. There were only three positives out of about 1200 horses tested, so the disease is still rare, but the fact that it was there and none of the positive horses had any link with previous outbreaks is definitely a concern. The OIE report states that transmission is suspected to have been from "artificial" means like sharing needles between horses, not natural tick transmission. This could account for the multiple horses affected but doesn't explain where the disease came from it the first place, and it's unclear how solid that hypothesis really is.

It's quite interesting (surprising, frustrating...) that few comments are put forth in any of these outbreaks indicating where the infections may have originated and why we are seeing recurrent, unrelated infections. Is increased testing in different areas helping to pick up cases that would otherwise have been missed (i.e. were already there), or is piroplasmosis in the US an emerging problem? How confident are they that there are no ticks capable of transmitting T. equi in some of these areas? Is wider screening of horses required to determine the extent of the problem and to determine whether it can be controlled? Is broader screening of ticks in the affected areas needed to see if there are ticks known to be able to transmit T. equi? Are studies needed of other tick species in areas where unexplained cases have occurred to determine if some tick species that are not currently known to be able to spread the parasite can actually do so? Lots of questions... hopefully someone's trying to find some answers.

Click image for source.

Equine Infectious Anemia In New Jersey

A pony in New Jersey has been diagnosed with equine infectious anemia (EIA) and euthanized. Details are pretty scarce at this point and it's unclear whether the pony was sick or whether EIA was simply diagnosed through routine testing. There's also no indication of where the pony might have acquired the infection, which is a very important question.

Equine infectious anemia is a viral disease of horses and other equids that is characterized by recurrent fever, lethargy, decreased appetite and decreased red blood cell numbers (anemia). Sometimes, the first episode of disease (acute EIA) can be fatal, but most horses recover. Most survivors experience recurrent episodes of illness. In some horses, these episodes become frequent and severe.

A major problem with EIA is that horses are infected (and are infectious) for life. Even if they recover from the acute disease and rarely or never get noticeable recurrent disease, they are still carrying the virus in their blood. Biting flies are the main means of transmission from horse-to-horse, although human-associated transmission through reuse of needles or other procedures that cross-contaminate blood between horses can also be involved.

The combination of life-long infection and an insect vector is BAD. That's why, in areas where EIA is not endemic, infected horses are usually immediately euthanized. In some regions, affected horses can be branded or tattooed to permanently indicate their EIA status and housed at least 200 yards from any other horse (and often with strict insect control measures).

Control of EIA focuses on identification of carriers. Most jurisdictions require regular EIA testing (formerly the "Coggins test") for horses that are traveling or competing, as well as imported horses. While this disease is extremely rare and the vast majority of routine tests are negative, it's a disease that can silently spread in a population if people are not looking for it - routine testing is critical for keeping this disease at bay.

Umbilical Care In Foals

As foaling season approaches, it's a good idea for people to review proper umbilical care. The umbilicus is an important route of infection in foals, and can be associated with problems including local umbilical abscesses, large abdominal abscesses extending to the liver, and overwhelming body-wide infection (sepsis). The reason the umbilicus is such a critical structure is that it contains three major blood vessels (two arteries and one large vein) and the urachus (which connects the umbilical cord to the foal's bladder) . When the umbilicus ruptures shortly after birth, these structures are exposed to the bacteria-laden environment of the outside world and can be a route of entry for local and deep infections. Care of the umbilicus during the initial high-risk period is a key part of raising a healthy foal.

Do all foal's need specific umbilical care? Not really. Most foals, especially those born normally in a clean environment to a healthy mare and who received adequate colostrum, don't need anything done. However, it's not always easy to differentiate these low-risk foals from others, and it is possible for the healthiest foal born in the cleanest environment with ingestion of an adequate volume of good quality colostrum to develop complications, so most people perform some form of post-birth umbilical care (and that's a good thing). The key is making sure that it's the right umbilical care.

The goals of umbilical care are pretty basic:

  • Prevent bacteria from entering the umbilicus.
  • Avoid damaging the umbilicus and other body tissues, and avoid delaying normal drying of the umbilicus.

What to use?

  • Research has indicated that a 0.5% chlorhexidine solution is the optimal umbilical dip. Other disinfectants can also kill local bacteria on the umbilicus but may not be as effective, may not work as well in the presence of debris (dirt, manure...), or may be irritating to body tissues.

Read the label:

  • Make sure you are actually using 0.5% chlorhexidine and that it's a solution (diluted in water), not a tincture (diluted in alcohol). If you don't have 0.5% chlorhexidine solution and are unsure about how to dilute it properly, ask your veterinarian.

More is not better!

  • Don't think that since 0.5% is good, 5% must be 10 times better. The stronger the concentration, the greater the chance of damage to local tissues, which can increase the risk of complications. Stick with 0.5%.

More is not better! Part 2

  • The umbilicus should be dipped in disinfectant, not marinaded in it! The goal is to cover the umbilicus and not other tissues (e.g. the abdominal wall). You don't need to soak the umbilicus or hold the disinfectant in place over it. Short term contact (dip) is adequate. Dip it and walk away. The umbilicus needs to dry up - repeated soaking isn't helpful.

More is not better! Part 3

  • The umbilicus should be disinfected shortly after birth, then every 6-8 hours for the first 24 hours of life. That's usually enough. If the umbilicus still appears wet at that time, it can be dipped again. Continued dipping "just is case" is not needed.

Don't tie off the umbilicus:

  • Tying off the umbilicus can actually increase the risk of complications such as infection and patent urachus (urination through the umbilicus).

Hands off!

  • Don't touch, poke or otherwise make contact with the umbilicus with your hands. It's not needed and it's a great way of transferring bacteria to the umbilicus.

If in doubt, call your veterinarian:

  • A proactive call to your veterinarian is much better and cheaper than an umbilical infection, umbilical abscess, septic foal or patent urachus. These are all expensive complications and  often difficult to treat successfully. Foals can change very quickly, and waiting to "see what happens" for a day or two can be the difference between a minor complication and a life-threatening problem.

UK Yanks Low-Risk Status For Canadian Mares

The UK's Horserace Betting Levy Board (HBLB) has published their 2010 Codes of Practice for prevention and control of equine infectious diseases.  As part of these codes, Canada and the US have lost their low-risk status for contagious equine metritis (CEM) because of the large 2008-2009 US outbreak of the disease (the HBLB calls it a US and Canadian outbreak, which isn't actually true). Canada is included presumably because of the number of exposed horses that entered the country (and the number of other unknown exposed horses that could have entered given the scope of the US outbreak) and perhaps the somewhat passive response by federal authorities in Canada.

The loss of low-risk status greatly affects how Canadian mares are handled upon arrival to breeding facilities in the UK, and will create major hassles for people moving horses to the UK for breeding. There's no indication whether this is a permanent change or whether it may be revisited with more information on the status of CEM in North America.

Image source: http://animalphotos.info/a/

Bug of the Month: Rotavirus

Rotavirus is an important cause of diarrhea in young horses. (It's also a major cause of diarrhea in infants, but a different rotavirus is involved). Exposure to rotavirus is very common - most, if not all, horses are exposed to it early in life. Disease only occurs in foals, but not every foal that gets exposed becomes sick. Most often, rotavirus causes diarrhea if foals between 1 and 6 months of age, with most cases occurring between 1 and 3 months of age. Foals less than 1 month of age can be affected, but it's less common.

Foals become infected by swallowing the virus, which they usually pick up from the feces of other horses (including their mares) in their environment. The exact source of infection is rarely identified. It is likely that some healthy adult horses are the reservoirs and shed the virus in their manure.

Like other types of diarrhea, rotaviral diarrhea in foals can range from mild diarrhea alone to severe diarrhea with weakness, loss of appetite and dehydration. Colic can occur because of intestinal cramping associated with diarrhea. You cannot differentiate diarrhea due to rotavirus from other types of diarrhea by appearance alone. The diarrhea must be tested to identify the cause.

There is no specific treatment for rotavirus infection, but "supportive care" is often provided. One of the primary components of supportive care is fluid therapy, which may be needed for foals that start to become weak and dehydrated. Aggressive intravenous fluid therapy may be needed in some cases, depending on the severity.

Rotavirus vaccines are available in some regions, although there is limited evidence that they have much of an effect. Few people recommend vaccination. Rather, good attention to foal management practices, hygiene and early diagnosis of infected foals is the key. Foaling mares outside on pasture has been an effective practice in some outbreaks, presumably because there is less rotavirus contamination on pasture.

Image: Colourized TEM of rotavirus particles (source: CDC PHIL 173)

US Piroplasmosis Outbreak Widens

A large number of infected horses has now been identified in association with the ongoing outbreak of piroplasmosis in the US. Three hundred seventeen (317) positive horses have been identified in 11 states: Texas, Alabama, California, Florida, Georgia, Louisiana, Minnesota, North Carolina, Tennessee and Wisconsin. Two hundred eighty eight (288) of the positive horses are from the index farm in Texas. All positive horses are under quarantine (and will likely be euthanized), and testing of other in-contact horses is ongoing.

This report is very concerning for several reasons:

  • A previous report indicates that positive horses were likely on the index farm for at least a year or two, making it likely that more infected horses are out there. The longer a disease like this goes unnoticed, the farther it can be spread before it's detected and controls are put in place. It's possible that an even larger number of infected horses are in the US now.
  • The widespread infection makes it more likely that the disease will establish a true foothold in the US. This bloodborne parasitic disease (caused by Theileria equi) is naturally transmitted by certain types of ticks (not all tick species are able to transmit the parasite). The more areas in which infected horses are found, the greater the chances that infected horses will be bitten by ticks that can transmit the parasite. If it gets established in the tick population, it becomes much harder to control.
  • It's not known how so many horses became infected. With this number of infected horses, it seems to me that tickborne transmission is more likely, rather than human-associated transmission through re-use of needles or other means of transmitting bloodborne pathogens. If the disease is being spread through it's natural route, it's harder to control.
  • It is still not clear where this outbreak (or the other recent US outbreaks) originated. If you don't know how something started, it's hard to prevent it from happening again.

Piroplasmosis may be on its way to becoming an endemic (established) disease in the US. Broad investigation is required to see if it is present beyond the affected premeses identified so far.

Image source: http://animalphotos.info

Piroplasmosis And The US: Let's Just Call It An Endemic Disease

Following on the heels of a few outbreaks of piroplasmosis in the US over the past year is a report of two piroplamosis-positive horses from New Jersey. This bloodborne parasitic disease is officially a foreign disease in the US, but the number of recent outbreaks and their unknown origin certainly suggest that this disease has a solid foothold in the US. The latest situation in New Jersey involves two horses purchased from the Texas farm which is currently under quarantine due to its involvement with the most recent outbreak of piroplasmosis in that state. However, these two horses were purchased in 2008, which strongly suggests that this disease has been in the Texas herd (and presumably elsewhere) since at least that time. Odds are this disease is actually wide-spread in some areas of the US.

Losing national piroplasmosis-free status could have significant repercussions on horse movement to and from the US, and a major impact on the equine industry overall. For this reason, some people might prefer to try to ignore the problem and hope it goes away. But, as I've said before, hope is not an infection control strategy.  It's much better to investigate this carefully and transparently to figure out what is going on. Without knowing the scope of the problem, it's impossible to control it.

Image source: www.australiasigns.com.au

Papillomavirus Part 4: Equine Canker

From Guest Author Dr. Angelika Schoster, Department of Clinical Studies, University of Guelph.

Equine hoof canker (pododermatitis chronica verrucosa) is described as a chronic, proliferative dermatitis, beginning in the caudal part of the cleft of the frog and gradually expanding to the sole and wall. Equine canker is not lethal in and of itself, but because of where it occurs on the foot, and because it can be so difficult to treat and it recurs so often, it can severely compromise a horse’s ability to do its job (even if that job is just walking around in a field sometimes). The etiology of equine canker has been a topic of discussion for over 50 years, but so far no specific cause for the disease has been found.

Canker is usually diagnosed based on the appearance of the affected horny tissue and a distinctly fetid odour. The definitive diagnosis has to be confirmed by microscopic examination (histopathology) of biopsies collected from the affected tissue.

Treatment of canker has always been a dilemma for veterinarians and farriers because it is so difficult. Treatments used today can range from surgical intervention (removal of the affected tissue) to conservative medical management using topical medications of many different kinds.

The proliferative but non-metastatic nature of canker is similar to that of equine sarcoids. Like canker, sarcoids also tend to be difficult to treat and often recur. Both canker and sarcoids often include a mixture of proliferative and erosive changes in the skin secondary to overgrowth and thickening of the tissues. Due to these similarities, it has been speculated that bovine papillomavirus (BPV) might also be involved in causing canker. A large study in Europe (which will hopefully be published soon) has found evidence to suggest that a variant of BPV-1 plays a role in the development and/or maintenance of canker lesions in horses. Much more study is still needed, but this is another interesting example of how an infectious pathogen may ultimately play a role in a disease that’s origin has remained a mystery for decades.

More information about papillomaviruses is available in our archives.

Image: Severe canker in the frog of a horse's hoof (source: www.answers.com)

Texas Piroplasmosis Update

The following report was provided by the Texas Animal Health Commission.

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November 4, 2009

Equine Piroplasmosis Disease Investigation Continues

Canada and a number of U.S. states have imposed movement restrictions or additional entry requirements for horses from Texas after equine piroplasmosis, a tick-transmitted blood disease of equine animals, such as horses, donkeys, mules and zebras, was detected in South Texas in mid-October.  Equine piroplasmosis may be carried and transmitted by as many as 15 species of ticks.  Although ticks have been collected from the South Texas ranch for testing, final results are not complete, and it is not known whether any of the ticks can serve as a host for the disease.

“Before moving horses from Texas, we urge you and your veterinarian to check with animal health officials for any state of destination, to ensure the animals have met all entry requirements,” said Dr. Bob Hillman, Texas’ state veterinarian and head of the Texas Animal Health Commission (TAHC), the state’s livestock and poultry health regulatory agency.  “Regulatory requirements can be fluid as disease situations evolve, so it is essential to call each state each time you haul.”  As states provide entry restrictions and requirements, the documents are posted on the TAHC web site at http://www.tahc.state.tx.us.  Dr. Hillman urged equine owners and veterinarians to call state animal health officials directly before hauling, as many states have not yet distributed entry requirement information. Contact information for state veterinarians may be obtained from the TAHC at  800-550-8242, ext. 710, or by emailing ceverett@tahc.state.tx.us.

“We are continuing the equine piroplasmosis disease investigation initiated in October in South Texas. No horse movement is being allowed from or to the ranch where the infection was detected,” said Dr. Hillman.  “While this tick-borne disease has not been considered endemic in the U.S., cases of the disease, scientifically known as Theileria equi, and previously called Babesia equi, have been detected in the U.S.   Our epidemiologists are tracing the movement of specific equine animals. Blood tests will be conducted, and the animals will be examined for ticks. Individual equine owners will be contacted, if their horse needs to be tested by animal health officials.”

Dr. Hillman refrained from speculating on how many equine animals will be tested or how many may be exposed or infected.  “Until the epidemiological work and testing of potentially exposed horses is completed, there is no way to predict how many horses may be affected with this tick-borne illness,” he said.

Dr. Hillman said horses infected with equine piroplasmosis may appear well, while others may exhibit a host of non-specific clinical signs, such as fever or anemia. These clinical signs also could be attributed to a variety of other diseases or causes. Blood tests are needed to diagnosis the disease. 

“Equine owners should talk with their private veterinary practitioners about complying with interstate movement requirements, testing recommendations and protecting their horses from ticks.  If a horse appears to be ill, it should be evaluated by an accredited private veterinary practitioner,” said Dr. Hillman.

Texas Animal Health Commission
Bob Hillman, DVM * Executive Director
For info, contact Carla Everett, information officer, at
1-800-550-8242, ext. 710, or ceverett@tahc.state.tx.us

We thank Carla Everett, Texas Animal Health Commission for sending this report.

Case Presentation: Neurologic Mare

A five-year-old Quarter Horse mare was found down in the field in the mid-afternoon.  The horse was seen moving around the field normally less than eight hours earlier.  She was found near a fence, but there were no external signs of trauma on the mare's body.  Some green feed material was present at the nostrils.  With encouragement the mare was able to stand, but she was very unsteady and uncoordinated, particularly in the hind limbs. Upon examination by the veterinarian in the field, it was also noted that the horse could not open her mouth normally (lockjaw - which is often a sign of tetanus in horses), and the mare seemed "dazed".  There were three other horses kept in the same field, all of which appeared completely normal.  All the horses were fed the same round-bale hay (a new bale was just put out the previous day).  The mare was vaccinated for rabies in 2008 and West Nile in 2009 (spring).  There was no movement of horses on or off the farm (i.e. the horses were not taken to shows/competitions or off-site rides).

When the horse arrived at the hospital, she was down on the trailer.  By that time she had a fever, high heart and respiratory rates, and she was dehydrated.  Although she was still aware of things going on around her, she was very depressed.  She was still able to see, and she could move all four legs and her tail.  However, it was even difficult for her to lie on her chest (e.g. sternal recumbency), so she would roll to one side instead (e.g. lateral recumbency).  Her jaw remained rigid, but she could still move her tongue a little.

With a lot of encouragement and help the mare was able to stand up and stumble off the trailer, but she was so weak and uncoordinated in all four legs that she fell down again before she could even walk the 10 metres to her stall.  Eventually she made it to her stall, and she was able to stand for about an hour before she lied down (or fell down) again.  A urinary catheter had to be used to drain the mare's bladder because she did not seem to be able to urinate on her own.

The mare was treated with intravenous fluids (supportive therapy), and anti-inflammatories and steroids to try to reduce the inflammation that was suspected to be going on in her brain and spinal cord.  Despite all this, her condition continued to worsen, and by the next morning the mare could not even sit up and was becoming less aware of her surroundings.  The mare was therefore humanely euthanized.

Post-mortem tests in this mare confirmed there was inflammation in the brain based on a high number of inflammatory cells in her cerebral spinal fluid (CSF).  Because of the clinical presentation, some of the brain tissue also had to be sent away for rabies testing, which was (thankfully) negative.  Once that result was back, samples were also tested for evidence of infection with equine herpes virus (EHV-1), West Nile virus and Sarcocystis neurona (the cause of equine protozoal myeloencphalitis), all of which were also negative.  Botulism was also considered, but this disease is very difficult to test for in horses.  In the end, the final diagnosis, and the cause of the mare's neurological signs, was infection with eastern equine encephalitis (EEE) virus .

This case of EEE was diagnosed in September 2009 in Southwestern Ontario.  The description of the presentation, and how rapidly this mare deteriorated, demonstrates just how devastating and severe this disease can be.    This case also tells us that there are mosquitos in the area that are carrying EEE.  Vaccination of the other horses in the region will not provide protection before the end of this mosquito season, but owners of horses in the same area should seriously consider (and discuss with their veterinarian) vaccination of their horses against EEE in the spring, prior to the next mosquito season.  No one can say for certain if vaccination of this mare would have prevented the infection, or decreased the severity of the infection, but it likely would have helped.  In the meantime, as always, decreasing exposure to mosquitos as much as possible (if there are any left) should be the priority.

Image: TEM of the salivary gland of a mosquito infected with eastern equine encephalitis (EEE) virus (source: CDC Public Health Image Library #7057).

Can The US Be Piroplasmosis Free?

The ongoing large piroplasmosis outbreak on a Texas farm, following on the heels of a few other US outbreaks, raises concern that this infection is not really a "foreign" disease in the US (at least not anymore). Although the source of the earlier outbreaks was not identified, they did not raise too much concern because ticks capable of transmitting this bloodborne infection were not found (which reduces the risk of uncontrolled spread of the disease). However, the situation may be different in Texas. Following identification of the first case, 31 more positive horses were identified on the affected farm. That's a lot of infected horses, and given the number it's probably less likely that the infection was spread by human activity (e.g. reusing contaminated needles) than by ticks. Concern has been expressed over the past couple years that Boophilus microplus and Rhipicephalus microplus, ticks capable of transmitting this parasite, might be establishing themselves in the state of Texas. If this is the case, this may not be an easily controllable situation, and the US may lose its piroplasmosis-free status. Intensive investigation of the source of this outbreak, how it was spread on this farm, whether there are other affected horses in the region and whether these tick species are present, is urgently needed.

Image: A "hard tick" (male brown dog tick (Rhipicephalus sanguineus)) from the same genus as R. microplus, one of the tick species capable of transmitting piroplasmosis (Theileria equi) to horses. (CDC Public Health Image Library #7646)

More Piroplasmosis In The US

For a country that is "piroplasmosis-free," the US sure has a lot of piroplasmosis. The Texas Animal Health Commission has reported that this bloodborne disease, caused by Theileria equi, has been confirmed on a ranch in south Texas. The farm is quarantined and testing is under way to determine the scope of the problem. They are presumably also looking at ticks in the area to see if the types of ticks that are able to transmit the infection are present, and trying to figure out where the infection came from.

This is one of a couple of recent outbreaks of piroplasmosis in the US. The source of infection in these outbreaks is typically not found, but careful testing and quarantine of animals is usually effective at containing the disease. Unfortunately, positive horses can be infected for life, and are therefore usually euthanized. Long-term isolation or shipping infected horses to a country where the disease is endemic are other options.

Interestingly, I've not seen any comments about whether this outbreak could be linked to the recent outbreak is Missouri, where some infected horses were taken out of quarantine and have disappeared. It's certainly logical to consider that one of the fugative horses may have been  smuggled into Texas and been the source of this latest infection. Hopefully this is a small, contained outbreak that doesn't result in widespread death of horses and economic disruption. Hopefully the source is identified promptly and measures are taken to reduce future problems. Only time will tell.

Papillomavirus Part 3: Sarcoid Stresses

From Guest Author Dr. Angelika Schoster, Department of Clinical Studies, University of Guelph.

Papillomaviruses are generally strictly species-specific, meaning a certain type of virus will only infect one animal species. Infection of horses with bovine papillomavirus is the only known example (so far) of cross-species infection within this group of viruses. In cattle, bovine papillomaviruses (BPVs) cause warts, just like equine papillomaviruses cause warts in horses. It is now widely accepted that bovine papillomaviruses (BPV-1 and BPV-2) are also the primary cause of equine sarcoids. More recently, these viruses have also been implicated in some cases of equine dermatitis and equine hoof canker (more on that in another post).

Equine sarcoids are skin tumours that affect horses, donkeys, mules and zebras. They are the most common type of tumour found in horses, and account for 35-90% of all equine skin tumours. Sarcoids are generally not life-threatening – unlike some other tumours, they do not metastasize (i.e. spread to other organs or tissues). However, they can be locally aggressive growths, meaning they may invade deep into the tissues immediately surrounding the primary tumour. They are also very difficult to treat, and therefore can affect a horse’s welfare or quality of life, depending on the size and location of the sarcoid. There is some evidence of a genetic predisposition to sarcoids in some horses as well. Involvement of bovine papillomavirus in the development of sarcoids was first suggested by Olson and Cook in 1951, who were able to reproduce sarcoid-like lesions in horses by inoculating them with tissue from bovine warts. Since then, researchers have used PCR to show that the vast majority of sarcoids contain BPV DNA, whereas normal horses and non-sarcoid skin tumours do not. Although BPV DNA has been found in healthy horses living in close contact with sarcoid-bearing animals, there is currently no evidence that BPV or sarcoids are transmissible horse-to-horse.

Six types of equine sarcoid are described based on their appearance: occult, verrucous, nodular, fibroblastic, mixed or malevolent. Each type tends to occur on different parts of the body, and they also differ in how they behave (i.e. how fast they grow). However, all six types share the tendency to recur and become more aggressive if they are disturbed accidentally (e.g. traumatized) or iatrogenically (e.g. surgically (but incompletely) removed). Unlike warts, sarcoids rarely disappear on their own (resolve spontaneously). Many different techniques have been used to try to treat sarcoids, including surgical removal, cryotherapy (freezing), carbon dioxide laser therapy, hyperthermia (burning), irradiation, photodynamic therapy, immunotherapy, and chemotherapy. Unfortunatley, to date there is no universally effective treatment for sarcoids (which is why so many different things have been tried and continue to be tried).

There is currently no effective vaccine against sarcoids, but knowing that a virus (BPV) is involved may ultimately allow researchers to one day develop a vaccine or other means of prevention. In the meantime, it probably wouldn’t hurt to keep your horse away from any warty cows that may be around.

Photo: A horse with recurrent aggressive sarcoids on the medial aspects of both forelegs (credit: M. Anderson)

Who Should Pay For Hendra Virus Research?

A proposed levy on horse owners to fund Hendra virus research has been met with opposition in Queensland. It has been suggested that a $25/horse levy in Queensland would provide needed funding for research into this rare but deadly disease, but this has been opposed by some vets and horse owners. One comment in response to the suggestion of a Hendra virus research levy is that the disease kills humans, so it should be publicly funded. However, Hendra only affects humans who have very close contact with horses, so that's a questionable argument. Also, medical research funding is certainly not overflowing, and the odds of a study such as this getting funded this way may be limited because it is so horse-oriented. I run into the same problem all the time with zoonotic disease research grants. Medical agencies don't want to fund it because it's too animal related, while animal agencies don't want to fund it because it deals more with human health.

Who should fund equine research? Should the government (i.e. all taxpayers) be solely responsible, or should some of the responsibility fall on horse owners, who stand to benefit the most from equine research? This is particularly true for a disease like Hendra that is very rare, currently restricted to one region, and only affects horses and people associated with horses. The rarity of the disease means that industry (e.g. vaccine companies) is probably not eager to fund research (because it would not be profitable). The focal nature of the problem geographically may limit interest from national or international groups. These factors could result in failure to do the necessary research to try to control this deadly disease.

This raises broader questions about funding for equine research. Many people and governments make lots of money from horses, directly or indirectly. You'd like to think that since so much money is made off the backs of horses (both figuratively and in some cases literally), that some of the profits would be put back into helping ensure the health and welfare of these animals. A fraction of a percent of the money generated by horses would be a tremendous asset for equine research, and help make great strides in improving the health and welfare of horses.  Unfortunately, such funding is rarely available, and equine researchers are often very limited in terms of the research that can be done with the available dollars. As a researcher, I know the difficulties of finding enough research funding to pay laboratory personnel and grad students, plus perform high quality research. The limited funding that is available is one reason that equine research is now only a fraction of my overall research program. The equine industry as a whole needs to think about its role in research, even if it's from a self-serving standpoint whereby research is funded to help boost performance and profits.

Rhodococcus Infects More Than Lungs

Rhodococcus equi is a bacterium that is an important cause of respiratory disease in foals. It typically causes numerous abscesses in the lungs, and it is an important cause of illness and death in foals from a few weeks to about six months of age. Infections of other parts of the body can occur, and these can cause serious problems because they can be hard to diagnose and hard to treat.

A recent study in the Journal of the American Veterinary Medical Association (Reuss et al 2009) described extra-pulmonary disorders (EPDs, disorders in parts of the body besides the respiratory tract). They studied 150 foals, and 74% had a least on EPD. These included:

  • Immune-mediated synovitis (25%): This is a well-recognized problem where R. equi-infected foals get enlarged joints because of accumulation of excess joint (synovial) fluid. Fluid-distended joints can occur with joint infections, but with immune-mediated synovitis it's caused by the immune system alone. This typically does not result in long-term joint problems, although it's important to determine whether the foal has an infection which needs aggressive care, or just inflammation which is best left alone.
  • Abdominal abscesses (17%): This is a very serious problem and abscesses in the abdomen can be very difficult to treat. These foals often die.
  • Uveitis (11%): Inflammation of the eye, which can occur as a result of the immune response or infection. 
  • Septic synovitis (9%): As opposed to immune-mediated synovitis, this is an infection in the joint. This is very serious and can cause permanent joint damage.
  • Pyogranulomatous hepatitis (11%): This is inflammation of the liver with abscesses and pus.
  • Pleural effusion (3%): Accumulation of fluid between the lungs and body wall. This can be the result of severe lung disease.
  • Meningitis (3%): Infection of the layer covering the brain, which is never a good thing.

Foals that had EPDs were more likely to die than foals with infections only involving the lungs, which isn't particularly surprising. In particular, the presence of uveitis, bloodstream infection, joint infection (not immune-mediated synovitis) and abdominal abscessation were associated with failure to survive.

EEE in Nova Scotia

A Nova Scotia horse has died of eastern equine encephalitis (EEE). EEE is a rare disease in Canada, but it is sporadically identified in some provinces. It was reported that this is the "first case" in Nova Scotia, although it wasn't clear if they meant in 2009 or ever.

EEE is a highly fatal mosquito-borne viral disease of horses. There is no specific treatment. The virus can also affect (and kill) people, but horses cannot transmit the infection to people -  horses and people (and some other species) are infected by being bitten by infected mosquitoes.

The veterinary clinic that diagnosed the case reported that 120 doses of EEE vaccine will be delivered there this week. A ProMed moderator commented that he/she hoped a major vaccination campaign will be started. However, it's far from certain that any vaccination efforts will be effective, particularly for this season. EEE is a very sporadic disease in Canada, but since it's so severe, vaccination certainly should be considered. However, considering most horses in Nova Scotia have never been vaccinated previously for EEE, it will likely take a couple of doses and several weeks before adequate antibody levels are present. By that time, the risk of exposure may be even lower because the mosquito season is ending in the maritimes. I'm definitely not saying don't vaccinate, however, because of the short remaining mosquito season and the lag time from vaccination to effective immunity, the immediate focus should really be on mosquito avoidance.

Deciding whether or not to vaccinate against EEE in areas where it is very rare and sporadic can be tough. From a population standpoint, it's somewhat difficult to recommend vaccinating all horses against a disease that may only affect a handful or horses, if any. However, from an individual horse standpoint, it's bad news if you happen to be that rare horse that gets infected. Really, it comes down to risk aversity and economics, and it's up to each horse owner (in consultation with their veterinarian) to determine what diseases they should vaccinate against.

US Piroplasmosis Outbreak "Resolved"

The recent piroplasmosis outbreak in Missouri and Kansas has been declared resolved. The concluding report has a few interesting findings:

Evidence indicates that in this outbreak, ticks, the typical vector of the disease, did not play a role in the transmission of the bacterium that causes piroplasmosis, Theileria equi. No infected ticks were identified and all ticks that were found on the farms were species that have not been shown to transmit the infection.

Disease transmission was attributed to poor management practices such as needle sharing between horses, which can result in transmission of bloodborne disease, including piroplasmosis. (Needle sharing is always a bad idea. There aren't too many bloodborne diseases in horses, but it's still stupid to reuse needles. They're incredibly cheap and there is no valid reason to do this).

The failure to find ticks that can transmit disease suggests that the risk peiod is over in the affected area. No one has explained, however, where this infection came from in the first place. That's an important issue that hasn't received a lot of attention.

There's also the sticky issue of the vanishing quarantined horses. Three infected horses were illegally taken out of quarantine and still have not been recovered. Therefore, "resolved" is a questionable term. The local outbreak is probably over, but these three escapee horses could still be a threat to the horse population wherever they are. The report states that unsubstantiated information indicates the horses may have been taken out of the country. That's obviously a concern for neighbouring countries like Canada.

Hopefully, this episode is really over. Hopefully the infected horses have been taken to an area where there are no ticks capable of transmitting disease. Hopefully people that have them are smart enough not to reuse needles. Hopefully the illegal and reckless action of sneaking out infected horses doesn't cause an outbreak elsewhere and result in the deaths of more horses. However, hope should not be an infection control strategy.

Is Hendra Virus More Common Or More Commonly Diagnosed?

Another horse farm in Queensland is under quarantine for Hendra virus. This follows the high-profile outbreak on another Queensland farm this past summer that resulted in the death of a veterinarian. Ongoing identification of affected farms is quite concerning for a disease that is typically very rare and sporadic. It's hard to say what this means.

Does it mean that Hendra virus is spreading and becoming more common? It's certainly a possibility. Even though we've known about this virus for a while and it's only caused rare outbreaks until now, established infectious diseases sometimes change their patterns for no clear reason.

Does this mean that Hendra virus is just being recognized more now? This is certainly a possibility too. Not all apparent changes in disease patterns are truly changes in disease patterns. Improved awareness, diagnostic tests or increased efforts to diagnose a disease can all lead to the impression that a disease is becoming more common when in fact it's just the same. I don't know whether that's the case in Queensland, but Hendra is certainly getting a lot more attention now.  I suspect that many horses that would not have been tested in the past are now being tested.

How do we answer these (and other) questions? Research and surveillance. We need to know a lot more about this virus. We need to make sure that horses with signs that might be indicative of Hendra virus get tested. Since veterinary medicine is a user-pay system and diagnostic testing often gets skipped (especially when a horse has already died), some form of subsidized testing might be required. Unfortunately, a lot of it comes down to money. Money is needed to do research, provide diagnostic testing, perform surveillance and educate everyone involved. Money for these types of efforts is getting harder to find, although it's usually much easier during a high-profile outbreak.

Image: Grey-headed Flying-fox (Pteropus poliocephalus) (source: www.environment.gov.au, photographer: Andrew Smith)

Ivermectin Toxicity With Appropriate Doses

A report in the September 1 edition (Swor et al, 2009) of the Journal of the American Veterinary Medical Association describes three horses with ivermectin toxicity. All three animals were adult Quarter horses from the same farm, and were among five horses that received a single tube of ivermectin paste (Bimectin) each. The tubes were the last in a box of tubes, the rest having been given to other horses on the farm with no problems.

Approximately 18 hours after all five horses were treated, three started to show signs of depression, drooling and ataxia (e.g. wobbly, unstable gait, poor balance). One horse was severely affected, and was sent to Texas A&M's equine hospital. The neurological abnormalities progressed in that horse, it became recumbent (i.e. unable to stand) and blind, and was euthanized. Nothing remarkable was detected on necropsy. Testing of the brain revealed an ivermectin concentration similar to the levels found in dogs with ivermectin toxicity. (Normally, ivermectin should not be detectable in the brain after deworming, because it should not be able to cross the blood-brain barrier.) The other two horses were less severity affected. They also ended up being hospitalized but recovered.

This is the first report of complications developing in adult horses treated with appropriate doses of ivermectin. Earlier reports of such complications have involved young horses receiving overdoses.

So what happened? A couple of possibilities were considered and dismissed:

Too much ivermectin in the product: Some residual paste was tested and it did not have excessive ivermectin levels.  Also, other horses treated with tubes from the same box were fine. Therefore, it's very unlikely that too much ivermectin was given.

A genetic sensitivity to ivermectin: All of the affected horses were unrelated.

The most likely explanation is compromise of the blood-brain barrier.  As mentioned above, the blood-brain barrier should keep ivermectin from reaching the brain, but if this important barrier was somehow damaged, then ivermectin could have reached the brain and caused the signs of toxicity. Although there was nothing to indicate that another condition was present that would affect the blood-brain barrier, this is still the most likely cause. The authors suspected that a toxin from a plant may have been responsible for weakening the barrier, such as toxicity from silverleaf nightshade. Some infectious diseases can also affect the blood-brain barrier, but there was no evidence of these. In the end, a definitive cause could not be identified.

This report shouldn't scare people away from using ivermectin or other dewormers. This is a rare complication from use of a very common drug. Rather, it should remind us that there are potential risks with virtually all drugs (including supplements), and that the cost-benefit of using them must always be considered. It's also another good reason to use dewormers prudently. Ivermectin toxicity should be considered in any horse that develops neurological abnormalities after treatment, and any potential adverse reactions should be reported so the extent of the problem can be determined.

Image source: www.horsechannel.com

Time To Improve 'Wussy' Equine Farm Infection Control

An Australian horse group, the Queensland Horse Council, is calling for the equine industry to change its culture in terms of biosecurity. I agree completely, however, as I've said before, I think the term "infection control" is really what we are talking about here. Biosecurity involves keeping pathogens off a farm, as is done with tight controls on pig farms. Infection control also tries to do this, but realizes it's not completely possible with how we manage horses, and therefore also focuses on controlling the impact and spread of infectious diseases on the farm.

This is obviously in response to the recent Hendra virus outbreak in Australia. It usually takes a remarkable (and sometimes tragic) event to get people talking about infection control. It becomes a hot topic, people call for improved efforts and resources, and everyone gets on board... for a few weeks. After a short period of time without problems, people tend to revert to their usual behaviour, and infection control often gets marginalized again. The key, therefore, is establishing a sustained effort. That's the hardest part of infection control, be it in a hospital or on a farm.

Queensland Horse Council (QHC) president Debbie Dekker is quoted in the Sydney Morning Herald as saying "We are a pretty gung-ho lot and biosecurity is treated like some sort of wussy thing. Everyone needs to tighten up their biosecurity practices."

Well said.

"We still have a lot of people who don't know about Hendra virus so we need to get the information out to horse owners."

Communication and education are key. Just telling people they need to do something is not going to be very effective. Infection control practices usually involve some degree of effort or change in normal practices. If people don't understand why they should change, they are less likely to do so.

Sporadic infectious diseases and outbreaks will continue to happen (in horses, people and others). We can never completely eliminate the risk. We can, however, reduce some of the risks. While nothing can change what has happened with the most recent Hendra virus outbreak, we should at least make sure some good comes out of it, and that it leads to improved infection control awareness in Queensland and beyond.

Thanks to Dr. Doug Powell of Barfblog for the headline.

Image source: www.qldhorsecouncil.com

Hendra Virus Vaccine: For Horses or People?

In the wake of the death of Dr. Alister Rodgers from Hendra virus, there have been increasing calls for the Australian government to put significant resources into Hendra virus research. Various areas need to be investigated, including how this virus is maintained in the bat population, how it is transmitted from bats to horses, ways to treat infection and ways to prevent infection. Vaccination is an obvious topic, and creation of a vaccine appears to be possible. However, as I wrote the other day, there's a question about whether a company would put millions of dollars into development of a Hendra virus vaccine for people, given that the disease is very rare, is currently limited to one region, and only appears to be a risk for people in close contact with sick horses.

One thing that needs to be considered is whether it may be better to develop a vaccine for horses rather than people. Think about it:

  • All reported human Hendra virus infections have come from people in close contact with sick horses.
  • Human vaccines are very expensive to develop, test, get approved and market.
  • Vaccines for animals are much cheaper to make because testing and regulatory requirements are not as strict. (This can lead to marketing of vaccines for animals with limited evidence of effectiveness, but the upside is that vaccines can get to market quicker and with less expense.)
  • People are often more willing to get their horses vaccinated than to get vaccinated themselves.

So, even though it might sound strange, development of a Hendra virus vaccine for horses may be a more effective way to protect people.

If this approach is taken, a key step would be continued research into the epidemiology of Hendra virus infection to investigate other routes of human exposure. If people can get infected by other routes, vaccination of horses obviously wouldn't address the entire problem. However, based on what we know currently, vaccination of horses might be the most effective, timely and economic response to this pressing problem.

Hendra Virus Claims Life Of Veterinarian

Unfortunately, Dr. Alister Rodgers, who had been hospitalized with Hendra virus infection acquired from a sick horse, died yesterday in a hospital in Brisbane, Australia. He was infected last month while treating an infected horse on a farm that was subsequently identified as having multiple horses infected with the virus. Despite experimental ribavirin treatment, he developed the infection three weeks later. He is the second veterinarian from Queensland to die from Hendra virus infection in a little over a year. Four of the seven people known to have been infected by this virus since it first emerged in 1994 have died.

Chickens, Campylobacter, Ulcers and Horses

What's the link between chickens, Campylobacter, anti-ulcer drugs and horses? Well, nothing direct but a recent paper in the journal Emerging Infectious Diseases provides more evidence that the use of proton pump inhibitors for treatment and prevention of gastric ulcers can be associated with increased risks of certain types of infectious diarrhea in people. People who used this type of acid-suppressing drug were more than 3 times as likely to have Campylobacter diarrhea than people who didn't. Biologically, it makes sense. The acid barrier of the stomach is designed to kill harmful ingested bacteria, and decreasing that acid barrier through the use of acid-suppressing drugs can help bacteria survive passage through the stomach and subsequently cause diarrhea when they reach the intestines. It's been reported as a risk factor for Clostridium difficile diarrhea as well.

But does this apply to horses? We don't know. Infectious diarrhea is certainly a problem in this species, but no studies have looked at whether anti-ulcer drugs like omeprazole (Gastrogard) are a risk in horses. It makes sense that they could be but there's currently no proof. The reason I've been concerned about this is that anti-ulcer drugs are sometimes used like candy, when there's no clear evidence that they're necessary. These are important drugs for certain horses and are crucial for treatment in horses with ulcers. However, a lot of other horses receive anti-ulcer drugs prophylactically, and it's unclear whether the possible benefits of ulcer prevention therapy outweigh the potential risks of infectious diarrhea.

Without any clear evidence, the key is to weigh the potential risks and benefits. If your horse has ulcers (especially severe ones like in the gastroscopy picture above), then it certainly should be treated. If it has recurrent ulcers that keep coming back, prophylactic treatment may be useful, but management changes should also be made if possible to reduce the risks. In horses that do not or have not had problems with ulcers, I'm not convinced that routine anti-ulcer treatment is needed (or even a good idea). In certain situations where the risk of exposure to an infectious cause of diarrhea is higher (e.g. diarrhea outbreak on the farm, admission to an equine hospital) or when the horse has other risk factors for diarrhea (e.g. antibiotic treatment), I'd be especially wary.

Remember: While anti-ulcer drugs are useful, they may have a downside, like most other drugs.  You need to think about both aspects (risks and benefits) when deciding whether to treat or not.

"Equine Strep" in People

I received the following question the other day: "I have a friend who had chemo embolization on tumor on liver in late June. She is in hospital now, and an abscess was discovered on liver.  Pathology results said "equine strep".  Her brother visited immediately after procedure, and he works with horse full time."

Streptococcus is a group of bacteria that includes many different species. There are two main species in horses Streptococcus equi subsp. equi (aka S. equi, the cause of strangles) and Streptococcus equi subsp. zooepidemicus (aka S. zooepidemicus, a cause of various types of infections). As you can guess by the 'equi' name, their natural host is the horse. Strep infections are very common in people, but rarely involve these two species. Nonetheless, infections with either Streptococcus equi or S. zooepidemicus can be found in people, but S. zooepidemicus is most common. Usually, these infections develop in people who are already sick for another reason, have compromised immune systems, or in young children. Interestingly, not everyone that is infected reports direct or even indirect contact with horses.

Back to the question: it's hard to say what's going on here based on the the general term "equine strep", but presumably the person has an infection with S. equi or S. zooepidemicus. Whether horses are actually involved will be tough (or impossible) to determine. It's a tempting hypothesis that the patient's brother carried the bacterium from the farm to the hospital, but I'd be wary about making a definitive statement about the bacterium's origin solely based on that. There are ways to investigate this further, such as trying to isolate Streptococcus species from horses on the farm, typing them and comparing them to the strain that caused disease in the person, but this type of testing is very costly and almost never performed, as human infection with these species is so uncommon.

This should be a good reminder that people who are sick and in hospital are at higher risk for developing infections, and they can get infections from bacteria that rarely cause disease in healthy individuals. While there is no proof of a link to horses (at least in this case), good infection control practices should be used whenever anyone visits someone in the hospital. That would include not wearing barn clothes to the hospital and paying close attention to handwashing.

Human Infection in Latest Hendra Virus Outbreak

A veterinarian, one of four people in Australia that were under close observation due to exposure to horses with Hendra virus infection, has developed signs of infection. Dr. Alister Rodgers is now in hospital in critical condition. He had close contact with a sick infected horse three weeks ago - Hendra virus was not considered initially, it was thought that the dying horse had been bitten by a venomous snake. Dr. Rogers didn't wear gloves or a mask when examining the horse because he had left them in his car.

Dr. Rodgers received experimental treatment for five days to try to prevent or reduce the severity of infection. He had returned home from hospital following the treatment only one day before he became ill. It has now been confirmed that he is infected. Only six people have been previously diagnosed with this rare disease; three have died. There were hopes that all of the exposed individuals would escape unscathed given initial tests showing no sign of infection and the experimental therapy, but it's clear now that early detection of infection is not easy.

Image: Coloured electron micrograph of Hendra virus (source: www.csiro.au/science/Hendra-Virus.html)

EPM Myths

To steal a line from Winston Churchill, equine protozoal myeloencephalitis (EPM) is a riddle, wrapped in a mystery, inside an enigma. It's a frustrating disease from many standpoints, and myths abound. A recent article from TheHorse.com highlights some of these myths.

One of the biggest problems with EPM is diagnosis. In Ontario, a large number of horses are "diagnosed" with EPM, but very few actually have the disease. Probably hundreds of horses (particularly race horses) are treated for every single horse that actually has EPM. Many horses get treated without any diagnostic testing at all, but merely based on vague (or no) signs that could be caused by numerous other conditions.

A key point in the article is the need for diagnostic testing and a reasonable suspicion of EPM before considering the diagnosis (and treatment). Unfortunately, none of the tests we have available are very good by themselves, which complicates matters. When you have a marginal quality test and you use it in horses that probably don't have disease, you end up with a lot of false positive results, which often results in unnecessary treatment.

According to experts in this article (with whom I absolutely agree), EPM should only be diagnosed if:

  • The horse has clinical signs consistent with the disease (this does not include vague, non-neurological signs like not running as fast as the trainer or owner wants)

        AND

  • A veterinarian has ruled out all other neurologic diseases that are similar to EPM

        AND

  • You have a positive EPM test result.

There are several testing options, each with good and bad points. There's not currently one "right" test that everyone should use. They key is SOME test should be used and it MUST be used in conjunction with a proper examination and other testing to rule out diseases like Wobbler's syndrome. Only when you are left with no other diagnoses and a positive EPM test should you really consider EPM likely.

There's a good statement at end of the article: "If it looks like EPM and smells like EPM ... it probably isn't. EPM remains a rare cause of neurologic disease in horses." EPM certainly does exist and can be a serious disease, but make sure you are not wasting time, effort and money treating a horse that doesn't really have it.

Pigeon Fever Warning for Colorado Horse Owners

Colorado State University veterinarians have issued a warning to horse owners in the state to be on the lookout for pigeon fever. They are reporting a spike in cases along the northern Front Range of Colorado.

Pigeon fever is a highly contagious disease caused by the bacterium Corynebacterium pseudotuberculosis. It is an important disease in areas where it's common, but it is essentially unheard of in other regions (I've never seen a case myself). This bacterial infection typically causes abscesses along the horse's chest, midline and groin. The condition can be very painful, and affected horses may be very lame or reluctant to move.  The infection also causes fever, lethargy and weight loss. In some cases, it can be fatal.

Corynebacterium pseudotuberculosis can live in the soil and enter the horse's body through wounds or other breaks in the skin. Flies, especially cattle horn flies, may also transmit the infection. People are not affected, but can transmit the bacterium on their bodies (e.g. hands), clothing or other items.  Despite the name, horses do not get the infection from contact with pigeons.

People in this region of Colorado (plus other areas where this disease occurs) should be on the lookout for affected horses. Initial signs are often vague and the diagnosis may not be apparent until the disease is already advanced. If you think your horse may be affected, contact your veterinarian as soon as possible. In the interim, your horse should be isolated to reduce the risk of further transmission. Areas on the farm where affected horses have been should be quarantined until they are adequately cleaned and disinfected - bacteria in pus from draining abscesses can survive for several weeks in the environment.

Image source: http://docsusan.blogsome.com/

Reporting Adverse Reactions

A recent post about mandatory vaccination in show horses sparked a discussion about concerns regarding adverse reactions to vaccines. Dr. Carolyn Cooper of the Canadian Food Inspection Agency posted the following comment.

"There is mandatory reporting of adverse vaccine reactions in Canada by the vaccine manufacturers to the Veterinary Biologics Section (VBS) of the Canadian Food Inspection Agency. VBS monitors the type and frequency of reports in order to identify any potential issues with the safety and efficacy of licensed products. In order to increase the usefulness of mandatory adverse reaction reporting, veterinarians should report all suspected adverse reactions to the vaccine's manufacturer. Any comments regarding the licensing of veterinary vaccines in Canada, or adverse reaction reporting in Canada, can be directed to VBS at http://www.inspection.gc.ca/english/anima/vetbio/conpere.shtml "

This is a very important reminder for veterinarians and horse owners. While there is mandatory reporting, adverse reactions to drugs and vaccines are rarely reported by veterinarians. There's nothing underhanded about the lack of reporting, it's mainly that people don't think about it when it happens. Without good data, however, we are left to debate the true risks of vaccines and drugs with only incomplete information. Drugs and vaccines can be marketed for animals with rather minimal safety data. Even with large studies, safety issues can be missed until the drug/vaccine is mass marketed, as has been shown with some high-profile human drugs. If regulatory agencies don't get the data (i.e. if the reactions aren't reported to them), then they can't adequately monitor safety concerns. If your horse has an adverse reaction to a pharmaceutical product, you should make sure this is reported. It doesn't take your vet much time and shouldn't take any effort on your part.

Rabid Horse in Maryland

A horse in Harford county Maryland has been euthanized because of rabies. The horse first starting showing signs of disease in mid-July, which manifested as "striking changes in behaviour." The report doesn't say when the horse died, but animals typically die within a few days of the onset of neurological disease. The horse was transferred to the New Bolton Center where rabies was diagnosed. Subsequent testing showed it was a raccoon rabies strain, although that does not mean that a raccoon was the actual source of infection.

Public health officials implemented a 45 day quarantine of the farm. Stray cats (about 25) were caught and euthanized. Fortunately, the family pets were properly vaccinated and have received booster shots (plus presumably a period of observation at home... a much better situation than if they were not vaccinated).

People that had contact with the horse have received rabies post-exposure treatment. This includes one person who had to be tracked down overseas.

Harford County Health Department spokesperson Bill Wiseman said "There was never a risk to public safety. This incident was a great example of public health work in action and cooperation between local, state and in this case, international authorities." I don't buy the statement that there was no risk to public health. While the risk of rabies transmission from infected horses is very low, it's not zero. Rabid horses have killed people because of their abnormal and sometimes aggressive behaviour. Further, the fact that this horse had rabies means that it got it from something. Rabies can have a long incubation period so it's not guaranteed that it acquired it on the farm, but you have to be prudent and assume that there is infected wildlife in the area that could pose a risk for other animals or people. Public health authorities managed the situation well and reduced the public health risks, but there were certainly still risks.

Rabies vaccination is highly effective. There is no statement about whether this horse was adequately vaccinated but it's unlikely. Proper vaccination would likely have prevented this horse's death, as well as the death of the stray animals, cost of vaccination of people, cost of veterinary care for this horse, quarantine of the farm and the associated financial and emotional costs. A dose of vaccine that costs a few dollars could have saved thousands of dollars and emotional stress.

Rabies is a rare disease in horses but its severity means it should not be ingored. Vaccinate your horses.

Hendra-Exposed Farm Personnel Get Experimental Drug

Four people exposed to horses infected with Hendra virus in Australia are taking an experimental drug to try to prevent or reduce the severity of infection. All are currently healthy, but it is unknown whether the virus is incubating in them and whether disease may develop.  People can be infected with this virus through close contact with infected horses, as was the case with these four individuals. While human infections are rare, 50% of infected people die. Therefore, it's understandable that they would choose to try an experimental treatment.

These people will be treated for five days with intravenous ribavirin, an antiviral drug. There is evidence that ribavirin can kill Hendra virus in the laboratory, but it's not known if it actually does anything in infected people. It has some potential adverse effects, but given the severity of disease and high risk that these people have been exposed, it's certainly a reasonable decision. This treatment was also used in the Hendra virus outbreak in 2008. One person died, one survived after a long stay in ICU, and one did not get sick.  It's not known whether the drug did anything to help. The death of the treated person doesn't necessarily mean the treatment is not useful for some people or for certain stages of infection. Hopefully, ribavirin has a better chance of working when infection is only developing, before these people get sick.

It's Back: Hendra Virus in Australia

Hendra virus, a virus that can kill horses and people, has resurfaced in Queensland, Australia. This bat-borne disease has caused periodic fatalities in horses and people that work with horses. The latest outbreak is thought to have killed up to three horses and resulted in the potential exposure of at least 30 people. The likelihood of these people getting sick depends on how close their contact was with the sick horses. Close contact with secretions from infected horses seems to be required to transmit disease. One person reported being snorted on by an infected, dying horse and being "covered" in blood, which is certainly concerning. An outbreak last year killed a veterinarian and hospitalized a veterinary nurse.

The farm in question is under quarantine and people that have been exposed are being monitored. There is no treatment for potentially exposed individuals, so they are in the unenviable position of having to wait and see if they get sick.

A virus like this is very hard to control. It's lives in fruit bats and only occasionally crosses into horses. The sporadic nature of disease makes it hard to control and predict when cases will occur. The key is early identification so that there is minimal exposure to other horses and people. People also need to take routine infection control precautions. One veterinarian handled an infected horse without using gloves or a face mask, as recommended, because he had left them in the car. Exposure to a potentially fatal infectious disease is not worth the few minutes of time saved by not following recommended precautions.

Photo credit: M. Anderson, 2009.

Equine Encephalitis Update: USA

The latest USDA report on equine encephalitis surveillance data gives a good picture of what has happened so far this summer with West Nile virus, eastern equine encephalitis (EEE) virus and western equine encephalitis (WEE) virus in the US. The data, last updated July 28, report no cases of WEE anywhere in the US. In contrast, EEE has been found in seven states (Florida (43 cases), Georgia (22), Mississippi (16), Alabama (6), North Carolina (4), Louisiana (3), Virginia (1)). This is a pretty typical distribution, with most cases being found in a small number of states, and even in certain regions within those states. West Nile virus cases are, not surprisingly, low so far. This disease does not tend to occur until late summer and fall. As of July 28, only six cases have been reported (Washington (2), one each in West Virginia, Texas, Montana and Missouri). The important question is what will happen with West Nile virus for the rest of the year? Stay tuned.

Image: Scanning electron micrograph of a mosquito (source: www.cbc.ca)

Ontario Equine Surveillance Report

Dr. Bob Wright of the Ontario Ministry of Agriculture Food and Rural Affairs has compiled a document with various pieces of information about disease occurences in horses in Ontario and Canada. While there's nothing too earth-shattering in it, it does give a nice synopsis of certain diseases and shows how Ontario is a pretty good place to be in terms of some nasty diseases. The report can be viewed by clicking here.

Canada Eases Import Restrictions

Earlier this summer, the Canadian Food Inspection Agency imposed quarantine restrictions on certain US states because of vesicular stomatitis. Texas is now (again) considered vesicular stomatitis free, since the previous cases have resolved and no new cases have been identified. Accordingly, import restrictions on horses coming from or through Texas have been lifted

Restrictions have been reduced for horses from New Mexico. Horses from non-infected counties can get import permits if they:

  • Have been inspected and had no signs of vesicular stomatitis within 10 days of export.
  • Have not been to or through an infected county within 21 days.
  • They are from a farm at least 25 km from any quarantined farm.
  • The have a negative ELISA test within 10 days of export.

(Image source: www.signs-up.com)

Piroplasmosis Makes Horses Vanish

TheHorse.com reports that a third horse that tested positive for the reportable, foreign disease equine piroplasmosis (Theileri equi infection) was illegally removed from quarantine in Kansas... in June. Two other horses were broken out of quarantine shortly after they were diagnosed in June, and have yet to be found. These horses could be anywhere spreading this disease, and may result in the US losing its piroplasmosis-free status. The latest OIE report indicates that the third horse was reported missing the day after the other two horses. That's one more potentially infectious horse on the loose, and one more threat.

This appears to be a good example of what can happen when you combine recklessness, carelessness and stupidity (perhaps with a little bit of laziness on the side). Any effective quarantine needs to be properly implemented and monitored. Any breaches of quarantine need to be immediately identified, reported, investigated and communicated. I haven't seen any previous information about the missing Kansas horse; I can't find anything in earlier press releases or other statements. If this information was withheld, it shows pretty blatant disregard for effective communications. People need to know when there are disease threats. Knowing that a horse with piroplasmosis is on the loose is important because this horse has to have gone somewhere - possibly a public stable or other facility with other horses. If people knew a horse was broken out of quarantine one night and someone showed up at the barn with a horse the next day, they could ask some questions or call the authorities to help protect their farm and their animals. Without this information, they're helpless.

(Image source: www.funnyphotos.net.au)

Still No Sign Of Missing Piroplasmosis Horses

There is apparently still no evidence regarding the location of two horses with piroplasmosis that were stolen out of quarantine in Missouri. I've been trying to find out more information, to no avail. TheHorse.com quotes a senior USDA veterinarian who states that, as of June 30, the location of the horses was still not known.

This is a pretty concerning situation. While piroplasmosis is not highly transmissible, these two missing horses could be putting many other horses at risk, as well as the piroplasmosis-free status of other regions of the US (if they have been moved to other states or infected other horses that have subsequently traveled to other states).

Piroplasmosis, which is caused by the bloodborne parasite Theileria equi, is transmitted by ticks and through contaminated needles.  If these horses are in an area where there are tick species that are capable transmitting this parasite, piroplasmosis could be silently spreading. Whoever took these horses, and anyone associated with this situation, are incredibly irresponsible and are putting many other horses at risk. This is another good reminder of why you need to be careful and ensure you know as much as possible about all horses (and their owners) that you allow on your property.

Parking Lot Quarantine For World Equestrian Games

International events like the World Equestrian Games are important and high profile competitions. They are also great places for disease transmission, with large numbers of horses from different areas brought together, along with associated stressors that can increase the risk of disease.

For the 2010 games at the Kentucky Horse Park, a long term parking lot at the Cincinnati/Northern Kentucky International Airport has been designated as a quarantine facility. Up to 600 horses will be temporarily stabled there after arriving from other countries. They will be required to stay in quarantine for at least 42 hours, to be monitored for signs of infectious diseases and for completion of selected laboratory tests. While it may seem strange at first, this site was chosen because it's readily accessible, close to the event, and can provide the needed security. Horses from some regions are subject to different quarantines at different facilities. For example, horses from Asia will be quarantined in Los Angeles, while South American horses are quarantined in Miami and tested for additional diseases such as Venezuelan equine encephalitis and screwworm.

Quarantine is a highly effective measure, if done properly, but it is not foolproof. You can never completely eliminate infectious disease risks because some diseases can have longer incubation periods than the quarantine, and others can be carried by healthy animals. Testing for specific pathogens can help pick up these hidden carriers, but you can't test for everything. You can never declare a horse to be free of all potential causes of disease, because such a horse doesn't exist. All horses carry something that could cause disease in certain situations, but in general these are low risk. The key is focusing efforts on the most common and most serious diseases.

Because quarantine and testing are not 100%, good general infection control practices are also required. The presence of a quarantine must not create a false sense of security and thereby be used as an excuse to relax basic infection control practices. These include things like restricting direct and indirect contact (e.g. sharing buckets) between horses, good hand hygiene by people working with the horses, and close monitoring for signs of disease, among other things. The intense preparations that are involved here show the importance of infectious disease in the equine industry and an excellent awareness of the problem.

EEE in Louisiana

It was only a matter of time, but eastern equine encephalitis (EEE) has been found in more US states this year. EEE is an seasonally important disease in some areas, including Louisiana. More cases in more states, and perhaps even some regions of Canada, are likely over the course of the summer and fall.

The latest case of EEE infection was reported in a horse in Rapides Parish, Louisiana. Not surprisingly, the horse died, as EEE has a very high mortality rate in horses - and in people.

If you live in a region where EEE cases have been identified or typically occur, you should have your horse vaccinated. Really, you should have already had your horse vaccinated, because it takes time for the vaccine to work after administration. However, even if your horse hasn't been vaccinated yet this year, get it done (better late than never).

Another important aspect of preventing EEE cases is mosquito control. EEE, like Western equine encephalitis and West Nile virus, is transmitted by mosquitoes. Measures should be taken to reduce mosquito populations in the area and mosquito exposure, both for your horse and yourself.

Vesicular Stomatitis In New Mexico

Not long after the first reported case of vesicular stomatitis (VS) of 2009 in Texas, the disease has now also been identified in De Baca County, New Mexico. It's not too surprising, because infectious diseases tend not to pay attention to state boundaries, and New Mexico tends to be one of the first states affected by VS during outbreak years. Reportedly, only a single horse has been infected in New Mexico so far, and the farm involved has been quarantined.

This will presumably result in many regions placing travel or import restrictions on horses from New Mexico, as was done with horses from Texas. Previously in some years when this disease has been detected in the US, it has been contained to a single state. During other years, multi-state outbreaks involving large numbers of horses have occur. Only time will tell what this year will bring.

People that live in or adjacent to areas where vesicular stomatitis is present should closely observe their horses for signs of this disease, including:

  • Drooling
  • Lesions in the mouth, ranging from raised, white lesions to blisters. After lesions rupture, ulcerated areas are present.
  • Swelling, inflammation and lesions around the coronary band (these are less common than mouth lesions).

This disease is primarily spread by insects, so direct contact with an infected horse is not required for disease transmission. Therefore, even closed herds need to be on the lookout. If signs consistent with vesicular stomatitis are found, movement of horses on and off the property should immediately cease and a veterinarian should be contacted as soon as possible.

Canadian Import Restrictions For Missouri and Texas Horses

The Canadian Food Inspection Agency (which, despite the name, is not only in charge of food inspection) has announced import restrictions on horses from Missouri and Texas. Horses that have been in those states in the past 21 days or who have passed through those states during transportation are being flagged because of concerns regarding exposure to piroplasmosis and vesicular stomatitis, respectively. Canadian horses traveling to those states will have to wait until the restrictions are lifted or will have to be moved to a non-affected state for 21 days before being eligible to come home.

If these two outbreaks spread beyond these two particular states, it's fairly certain that these restrictions will be expanded.

There's still no word on the location of the two horses that were "busted out" of their piroplasmosis quarantine in Missouri. It's surprising how little information as been forthcoming on this matter.

FBI Chasing Piroplasmosis Quarantine Escapees

TheHorse.com reports that two horses from Missouri that tested positive for equine piroplasmosis were removed from their quarantined stable without authorization on Wednesday night. Quarantine and testing of horses on the farm were implemented following identification of piroplasmosis in a Quarter Horse at a Missouri equine clinic. Five other positive horses were also identified and were euthanized with their owners' consent. The only other alternatives for piroplasmosis-positive horses in the US are long-term quarantine until negative or shipping them to a country where the disease is endemic. The Missouri Department of Agriculture, local officials and FBI are investigating.

Taking horses from a reportable disease quarantine is incredibly stupid.

Firstly, someone's going to notice. The authorities know what horses are quarantined and who owns them. No one has said who is suspected to have cut the padlocks to get at the horses.  I'm not saying that it was necessarily the owners who did it, but who would go to such effort to break an infected horse out of quarantine? I don't imagine they thought that the FBI would be involved.

Secondly, the horses are not being quarantined just because someone's trying to give the owners a hard time. This is being done because of the presence of a severe disease that is not normally present in the US. While it's not highly transmissible, the fact that multiple horses on this farm were postiive certainly shows that transmission can occur, be it through the appropriate tick vectors or other routes (e.g. contaminated needles).

Let's hope the people who took the horses come to their senses or get caught soon, so that the disease is not spread further. I assume that if there are other horses stabled where the escapees are eventually found, that those horses will need to be quarantined as well. I wonder if the people who might be harbouring the fugutive horses have thought of that.

Intramuscular Injections and Clostridial Myonecrosis

Clostridial myonecrosis (also called clostridial myositis or gas gangrene) is a very serious infection caused by growth of Clostridium bacteria in muscle. Clostridia are anaerobic spore-forming bacteria, meaning they only grow in environments without oxygen and they can stay dormant in the form of very tough (e.g. difficult to kill) spores. Clostridal spores can be found in healthy muscle. Presumably they enter the bloodstream periodically from the intestinal tract, but aren't able to grow because of the presence of oxygen in healthy muscle. They lie dormant, waiting for the right conditions to start growing - which in most horses never occurs. Clostridial spores can also be introduced into muscle during injections (e.g. vaccines, medications), surgery or trauma.

If clostridial spores are in a muscle and an anaerobic (oxygen-free) environment is created, they can start to grow. An anaerobic environment may form if the muscle is significantly damaged, such as from intramuscular injection of an irritating drug, or serious trauma. When the spores start to grow, they can cause very serious infection. Various clostridial species can be involved, but Clostridium perfringens is most common. Regardless of the particular species, infection is characterized by a very rapidly progression of soft tissue swelling, pain and systemic signs like fever and toxemia. The affected area can expand very quickly. Clostridia often produce gas when they grow and crepitus (a crackling feeling) can be felt over the infected area from the presence of gas in the tissues.

Clostridial myonecrosis is a life-threatening disease. It requires immediate and aggressive care, but is often fatal even with treatment. One of the most important (and dramatic) aspects of treatment is opening up the infected area to provide drainage and allow air (and oxygen) to enter the tissues. Large incisions need to be made into the muscle (this procedure is known as a fasciotomy). While this can look horrific, it's a critical aspect of treatment. High doses of antibiotics are also needed, along with other supportive care.

Fortunately, clostridial myonecrosis is relatively uncommon. Avoiding this disease involves decreasing the chances of creating the anaerobic environment in which clostridial spores can grow:

  • Intramuscular injection of irritating substances should be avoided. Flunixin meglumine (also called Banamine and a few other trade names) is commonly associated with this disease and should not be injected intramuscularly. Other irritating drugs include phenylbutazone (bute) and ivermectin.
  • Any muscle soreness or swelling that develops after an intramuscular injection should be taken seriously. A veterinarian should be contacted promptly, especially if the size of the affected area is rapidly expanding.
  • There is no evidence that any pre-injection techniques (e.g. swabbing the area with alcohol) have any influence on the occurence of this disease. Clostridial spores are resistant to alcohol, and most other disinfectants.
  • If your horse has clostridial myonecrosis, it should be treated at an equine hospital (if that's an option) because of the intensive care that is required. Aggressive and expensive treatment is needed. The prognosis is already guarded, but if optimal treatment isn't started promptly, the prognosis is very poor.

More information about clostridial myonecrosis is available on the equIDblog Resources page.

Vesicular Stomatitis In Texas

Vesicular stomatitis (VS) has been identified in a horse in south Texas. This viral disease is highly infectious and is a concern for various reasons.

Horses are often the first animals affected when an outbreak of VS develops. A quick response can help prevent the virus from spilling over into other species. It's a particular concern in cattle and sheep because VS can look very similar to foot and mouth disease, a tremendously important foreign disease that can have a devastating economic impact (just ask anyone from the UK).  Even though it is not usually fatal, vesicular stomatitis can also result in severely decreased production in food animals, and therefore also have a significant economic impact.

The impact of VS on infected horses varies. It causes painful blisters (most commonly on the upper surface of the tongue, surface of the lips and around nostrils, corners of the mouth and the gums) which can limit the use of the horse for a period of time (although infected horses can't compete anyway because the must be strictly quarantined). Some horses may be too sore to eat or drink normally, and therefore require supportive care. In unusual situations, the lesions are so severe that euthanasia is elected.

Vesicular stomatitis is a reportable disease in the US, and identification of this case has prompted a rapid outbreak investigation and response. The last outbreak, in 2006, was contained to 17 horses and 12 cattle in Wyoming. A much larger outbreak in 2005 infected livestock on at least 445 premises in 9 states. To help keep this virus contained, restrictions on livestock movement are promptly implemented. Infected animals and their herdmates are quarantined, neighbouring farms may be investigated and/or quarantined, and people are put on the alert to look for more cases. A fast response and cooperation of horse owners is critical. A major concern is  that people may have horses that develop vesicular stomatitis but don't tell anyone because that want to avoid being quarantined. This type of situation can prevent containment of the problem and lead to ongoing transmission and even bigger problems in the long run. Good communication and cooperation are essential.

Image from: http://www.newsinfo.colostate.edu

Eastern Equine Encephalitis in Georgia

Two horses from different farms in the Savannah, Georgia area have been diagnosed with eastern equine encephalitis (EEE). This is a severe neurological disease that can strike very quickly, and is fatal in up to 90% of cases. There is no specific treatment, and affected horses often deteriorate rapidly and dramatically. The virus that causes EEE is transmitted from birds to horses by the bites of infected mosquitoes, which makes it a seasonal disease. The risk of EEE is also heavily influence by geography, largely due to the distribution of mosquito species that effectively transmit the virus. Some areas have recurrent (and often major) problems with EEE, other areas (like Ontario) have few or no cases on a yearly basis, but can experiece small clusters of disease from time to time, and still other areas never see cases at all.

Identification of EEE is important for a few reasons. Obviously, it's relevant for horse health. It indicates that EEE virus is in the area and mosquitoes capable of transmitting it between birds and horses are also present. Horse owners need to be aware of the EEE history in their area when deciding whether or not to vaccinate against this deadly disease. On a seasonal basis, waiting until the first cases are diagnosed to vaccinate isn't a good plan, because there is always a delay from vaccination until peak immunity, and your horse could be infected before the vaccine is given or takes effect. EEE vaccination is best performed about one month prior to the at-risk period/season, with a booster six months later if the risk of exposure is still present (e.g. in southern areas were mosquitoes are present year-round).

Identification of EEE also has implications for human health. EEE can also infect people and cause very serious (and often fatal) disease. People cannot acquire EEE from horses, but hey are infected the same way: from the bite of an infected mosquito. A vaccine is not available for people so mosquito avoidance is critical.  Identification of cases of EEE in the area should be a reminder to look at farm measures to reduce mosquiotes and decrease mosquito bites (more information on this is available in an earlier post about West Nile virus).

Piroplasmosis In Missouri

Equine piroplasmosis, a foreign reportable disease, has been identified in a horse in Missouri.  On June 2, the affected horse (a seven-year-old Quarter Horse) was presented to an equine hospital with signs consistent with a bloodborne disease (although the exact signs have not been reported). The horse was isolated because of the potential for piroplasmosis, and an investigation was started. The sick horse and other horses from the same farm were examined for ticks (the vector of piroplasmosis) and none were found.  On June 10, the diagnosis of equine piroplasmosis was confirmed. The other 63 horses on the farm are currently being tested, the results are pending.

No source of infection has yet been suggested. The US was considered free of piroplasmosis after the last horse in a Florida outbreak cleared the infection earlier this year. This newly affected horse was apparently purchased six months ago, but no information was provided about where it came from. The lack of a clear source of infection is concerning because it could indicate that there are unknown cases somewhere else in the US.

Piroplasmosis is a tickborne disease cause by the protozoal parasite Theileria equi. It can cause signs of illness such as fever, anemia (decreased red blood cell count), jaundice, respiratory signs, reddish urine and weight loss. Up to 20% of affected horses may die. The parasite is naturally transmitted by ticks. It can also be transmitted through the re-use of needles or other blood-contaminated medical equipment, and perhaps through breeding if the semen contains blood. One problem with identification and control of piroplasmosis is that many infected horses show no signs of disease. Further, horses that recover can carry the parasite for prolonged periods of time and become long-term sources of infection for other horses, if the appropriate ticks are around to transmit the pathogen. Horses that are carrying this parasite therefore need to be strictly quarantined. Horses that become persistent carriers of piroplasmosis need to  be quarantined for life, euthanized or sent to a country where the disease is endemic. More information about the source of infection in this case and how far it has spread is anxiously awaited.

Malignant Catarrhal Fever In A Horse

An article in a recent edition of Emerging Infectious Diseases describes a case of malignant catarrhal fever (MCF) in a horse. MCF is a severe (usually fatal) disease caused by ovine herpesvirus type 2 (OvHV-2) or alcelaphine herpesvirus type 1. It mainly affects cattle - horses have traditionally been considered to be resistant to these viruses, but this recent report from Brazil challenges that belief. It describes a six-month-old foal with severe neurological disease that died one day after signs of illness were first noted. When veterinary pathologists examined tissues from the foal, there was severe damage to small blood vessels in the tissues (lympho-histiocytic fibrinoid necrotizing vasculitis... pathologists like using long names!) that was strikingly similar to that seen with MCF in cattle.

Sheep and goats are the main reservoirs of OvHV-2, and this foal came from a farm that also had goats. Molecular testing was performed on tissues from the foal and OvHV-2 DNA was identified. This virus was also detected in the blood of three adult horses (all healthy) and eight goats on the farm.

This was an interesting and quite a thorough investigation that challenges current dogma about MCF and horses. It's hard to say whether horses have always been slightly susceptible to the OvHV-2, with rare cases of disease going undiagnosed, or whether this is an emerging problem. It's unlikely this is a "hot" strain of OvHV-2 that can now infect horses, because other horses on the farm were fine and there have been no other reports of disease in horses. It's possible that this foal had some underlying problems that made it susceptible to infection by a virus that normally does not cause disease in this species. We certainly shouldn't panic about MCF based on this study and suddenly start keeping all horses away from sheep and goats.  However, this case should be a reminder that infectious diseases like to challenge conventional wisdom, and that we need to be on the lookout for new diseases and different patterns of old diseases.

Papillomavirus Part 2: Plaque Problems

From Guest Author Dr. Angelika Schoster, Department of Clinical Studies, University of Guelph.

No, not dental plaque like you get on your teeth - in this case we’re talking about aural plaques, which are a type of skin lesion that some horses develop on the inside surface of their ears. These plaques are usually depigmented, meaning the skin cells of which they are made do not have the same pigment in them as the cells of the surrounding skin, making the lesions appear pale grey or off-white. Other than being considered unsightly in some cases, aural plaques usually don’t cause problems for the horse – they’re not itchy, sensitive or painful. They can occur in any horse, no matter the age, breed or sex. Occasionally some horses may develop similar plaques on the udder or around the anus or vulva (under the tail). The diagnosis of an aural plaque is typically made based on the horse’s clinical signs alone (i.e. a raised, depigmented skin lesion inside the ear that is not associated with any inflammation or discomfort).

The exact cause of aural plaques is still not clear, but using extremely high-powered electron microscopy, researchers have been able to demonstrate that in some cases there is a papillomavirus infecting the cells within these plaques. No one has yet been able to isolate the virus to determine what type of papillomavirus it might be. Aural plaques are not known to be transmissible from horse to horse, but since there may be a virus involved, it's prudent to consider the possibility that virus transmission may play a role in their development (although there are likely many other factors involved as well).  Unlike regular papillomas (i.e. warts, caused by equine papillomaviurs), aural plaques do not disappear on their own. It the past, the only way to remove the plaques was to actually cut them away surgically. In more recent years, successful treatment has been reported using an immune-response modifier cream such as imiquimod (Aldara), which is used in people to treat several different skin conditions, including genital warts.

Photo: Aural plaque on the ear of a horse (source: University of Minnesota - College of Veterinary Medicine)

Papillomavirus Part 1: Wart Worries

From Guest Author Dr. Angelika Schoster, Department of Clinical Studies, University of Guelph.

Papillomaviruses (PVs) are small DNA-based viruses that are recognized causes of disease in many animal species and humans. More than 100 different human papillomavirus types (HPV) have been identified, which can cause a range of problems from annoying (but benign) warts to malignant cervical cancer. The ability of papillomaviruses to cause cancer was first recognized in animals, specifically with cottontail rabbit papillomavirus (CRPV), bovine papillomavirus (BPV) and canine oral papillomavirus (COPV). In general, PV infection causes benign disease in the vast majority of individuals, but the viruses have the potential to cause malignant disease in a small proportion of the mammals they infect as well.

Horses can be infected by both equine papillomavirus (EPV) and some bovine papillomaviruses. Infection with BPV is associated with equine sarcoids – look for more information on BPV in horses in an upcoming post. Infection with EPV (specifically Equuus caballus papillomavirus types I and II) causes cutaneous papillomas – small proliferative skin lesions better known as warts. Warts are benign growths that appear on animals less than two years of age. They typically persist for 6-12 months and then disappear without any specific treatment, and they do not otherwise make the animal sick. Usually warts appear on the muzzle and lips, but rarely genital warts can occur (usually with infection by the type II virus). Occasionally warts can also occur on the lower limbs, ears and eyelids.

Warts can be spread between horses by fomites (e.g. objects, equipment, tack, clothing that has been contaminated with EPV), or by close horse-to-horse contact. Spread is common when young are horses are brought together in large groups for shows, sales or breeding. Infection is usually diagnosed by the characteristic skin lesions, the horse’s history and age - more than 90% of affected horses are less than three years old. Usually the warts regress spontaneously and they do not require treatment. However, if treatment is sought for esthetic reasons, cryosurgery (i.e. freezing, usually with something like liquid nitrogen) can be performed. Caustic chemicals such as trifluoracetic acid can also be used to “burn” the warts off. Even after treatment, though, the warts can recur.

Controlling warts caused by EPV is fairly simple: if you have an affected horse, keep it away from other young horses. Do not allow the horses to have direct contact, and make sure the horse with the warts has its own tack, grooming supplies, water bucket, hay net/trough etc. so the virus is not indirectly transmitted to others. There is no vaccine available for horses for EPV. There are a lot of “voodo” treatments for warts out there, and some people swear by them, but (just like people who treat foal heat diarrhea) that’s usually because they’re treating a condition which goes away on its own anyway. People cannot be infected by EPV.

Photo: Warts on the nose and lips of a horse (source: http://www.yourveterinaryclinic.com)

CEM Update

The Horse.com reports that the ongoing contagious equine metritis (CEM) investigation in the US has now identified more than 820 exposed or positive horses. (Note the important difference between potentially exposed and positive - the vast majority of exposed horses will end up being negative). The expanse of this investigation is impressive, involving horses in 48 states.  The investigation is taking a long time because of the prolonged testing required to determine that exposed horses are indeed negative for the causative pathogen, Taylorella equigenitalis. This testing is also quite expensive, which make this disease a significant burden on the system even when only a few horses are infected.

Not a lot of new information has come to light recently. Hopefully that's a sign that the outbreak has finally been contained. Unfortunately, it's now known that some of the infected horses were positive for quite some time (years), and tracking all potentially exposed and infected animals over such a long period of time can be very difficult. The most recently diagnosed case was a stallion that was infected in 2006, if not earlier. The potential for spread of the disease, therefore, is still concerning, although two months have passed since the last new case was reported. The efforts to test exposed horses, costly and time-consuming as they are, are crucial due to the potentially even more costly consequences if CEM becomes established throughout the US (and therefore, almost inevitably, in Canada).

More information about CEM and the US outbreak can be found on the USDA website.

African Horse Sickness: US Outbreak Scenario

The latest edition of Equine Disease Quarterly, a newsletter produced by the Gluck Equine Research Center, has a couple of interesting articles. Coincidentally, one involves African horse sickness (AHS), a disease foreign to North America that I've mentioned in the past week. The article is about the potential threat of AHS being introduced into the US, and  gives a relatively brief but excellent overview of the disease and why there is concern. The authors discuss a hypothetical outbreak scenario in the US, which is certainly not beyond the realm of possibility - many of the required pieces to the puzzle are already in place when in comes to the potential for this virus to spread in the US:

  • The US has a large and often concentrated pool of susceptible horses. Very few horses have any immunity against the AHS virus.
  • Many areas of the country have weather conditions that are suitable for survival of the insect vectors (Culicoides midges) that might be introduced carrying the virus.
  • A different midge species, Culicoides sonorensis, is widespread in the US (except for the northeastern part of the country) and has been shown experimentally to be a highly capable vector for the AHS virus.

So what is needed for an outbreak to occur?

First, the virus needs to enter the country. This could occur by 1) inadvertent introduction of an infected midge (e.g. a "hitchhiker" on a plane would be one way for this to happen) or 2) introduction of an infected reservoir host. The reservoir host for this virus is zebras - fortunately  few zebras get imported into the US because of successful captive breeding programs, so this is probably less likely than the first. The other possibility, which certainly can't be ignored, is 3) intentional introduction of the virus: bioterrorism.

For an outbreak to maintain itself, a large population of reservoir hosts is needed. In Africa, the virus resides in zebras. Midges get infected by feeding on infected zebras, then infect horses by biting them. The lack of a large zebra population in North America could be the difference between a minor introduction and a major outbreak, unless there is another species that can act as a reservoir host in North America. This is one of the crucial unknown factors in the equation, but we shouldn't count on the lack of zebras as our main line of defense against this devastating disease.

The April 2009 edition of Equine Disease Quarterly can be downloaded here.

Image from: http://www.vet.uga.edu/vpp/fad/horse/vector.htm

Canadian Breeders and CEM Hassles

Canadian horse breeders are calling for efforts to streamline semen importation requirements that have been implemented by the Canadian Food Inspection Agency in response to the "outbreak" of contagious equine metritis (CEM) in the US. The new regulations and increased paperwork are causing tremendous problems for breeders trying to import semen in a timely manner. Any delays in clearing semen shipments at customs can result in decreased viability of the semen as well as problems scheduling personnel to perform insemination of the recipient mares. Sometimes, samples can't get through at all. Breeders shipping semen from the US must get health papers (sometimes from someone a few hours away) to send with shipments. Many of the challenges are simply logistical and bureaucratic problems that should be able to be addressed with some common sense and willingness to help. Regulatory and industry personnel need to figure out ways to address these problems without compromising the health of Canadian horses. 

While important regulations should be reviewed to see if they can be more practical and efficient, it is critical that biosafety not be sacrificed for convenience. The impact of a single case of CEM in Canada would be tremendous. It's increasingly clear the the CEM situation in the US is not completely understood.  It is not a readily definable outbreak with a clear source that can be tracked. Rather, this disease appears to be have been present in the US for a few years (at least), and that efforts to get the situation back under control will be very complicated. A "loose" effort in Canada could have disasterous effects.

Many equine veterinarians are concerned that the regulations currently in place are not adequate to prevent CEM from entering Canada. One major concern is the failure to evaluate horses moving across the border. The current regulations only deal with horses from farms where CEM has been diagnosed - it's far from certain that US authorities know where all the CEM-infected animals are. Since transmission of CEM is more likely during live breeding than artificial insemination, horses from these farms are a big concern. This is also potentially a situation where well-intentioned rules could have the wrong effect. Some people are shipping their horses to the US for breeding, because sending them down and bringing them back up doesn't require any extra precautions for CEM if the farm is not a "known" CEM farm. If there are some breeding farms that are infected but not identified, which is definitely possible, then the unregulated movement of these breeding animals could create a very hazardous scenario.

Equine Encephalosis in Israel

Coincidentally, just after writing my post on the concerns about the potential effects of climate change on diseases like African horse sickness (AHS), ProMED-mail posted a report about equine encephalosis (EE) virus in horses in Israel. This virus was first identified in South Africa in the 1960's. The most common signs of illness associated with the EE virus are high fever, decreased appetite, swelling (edema) of the lips and eyelids, and high respiratory and heart rates. Neurological disease and abortion can also occur. Most horses recover uneventfully with supportive treatment (i.e. no antiviral medications) but fatal infections can occur.

Equine encephalosis virus is an orbivirus like the virus that causes AHS.  The EE and AHS viruses are transmitted by the same type of insects - midges of the Culicoides family. As discussed in my other recent post, there is growing concern that AHS will re-emerge in Europe because the closely related disease of ruminants, Bluetongue, is now common in some areas that were previously free of the disease. Since all three of these diseases are closely related and involve the same insect vectors, it is logical to conclude that there may be a risk of both AHS and EE viruses reaching Europe, potentially resulting in widespread outbreaks, and eventually establishing themselves as endemic diseases.

Image from http://www.nationalinsectweek.co.uk/gbbu.php

Climate Change and Equine Diseases

Climate change is a pressing topic these days. There are many potential ramifications of climate change, including shifts in disease patterns. The climate affects the populations of insects that can transmit infections. This can result in expanding ranges of certain diseases, or movement of diseases into totally new regions.

Climate change has sparked significant concern in the UK about African horse sickness (AHS), a highly fatal disease that is transmitted by biting insects called midges (Culicoides species). Concern has been increased by the emergence and spread of Bluetongue virus in cattle and sheep in Europe. This virus is related to the AHS virus and is transmitted by the same insect species.  African horse sickness was identified in Europe (Spain and Portugal) in the late 1980s, but was eradicated from the region using aggressive programs including slaughter of infected animals, movement restrictions, vector (insect) control and horse vaccination. With the current insect populations and climate conditions, the concern is that if (or more likely when) the AHS virus reaches Europe again, it might be much more difficult to control.

Emergence of "foreign" infectious diseases can cause major problems. Almost no horses have antibodies against foreign diseases, because they've likely never been exposed to them.  This results in a large, highly susceptible population and an ideal scenario for a major outbreak. This was clearly demonstrated when West Nile virus found its way to North America, and when equine influenza virus reached Australia. Another problem is that few veterinarians know much about exotic diseases - most people don't spend much time studying or thinking about diseases that they don't think they are ever going to see. That can delay recognition of emerging problems.

Adequate response to foreign disease threats requires:

  • Good communication between horse owners, veterinarians, specialists and government officials.  Something we don't have.
  • Ready access to specialized diagnostic testing. This is variably available... but who pays for it?
  • Skilled government officials with a mandate, interest and expertise in equine infectious diseases.  Such people can be hard to come by.  Horses often fall to the wayside, as regulatory agencies tend to focus on food animal diseases and zoonotic diseases like rabies.
  • Willingness by horse owners and regulatory personnel to take early aggressive measures that may be required to contain a problem: I'm not convinced we have this, either.

It's impossible to be completely prepared for the unknown, but we know enough about foreign diseases and infection control to make a plan - to develop appropriate early intervention strategies to help contain outbreaks when they occur and try to limit their impact. Unfortunately, we don't have a good system to do this at the moment, and we are at high risk for problems should a foreign equine disease reach Canada. Unfortunately, it's a question of when, not if, this will catch up with us.

Preventing Catheter Site Complications

In equine hospitals, intravenous (IV) catheters are often placed in the large jugular vein in the neck of horses to make it easier for everyone (the horse included) to give the animal fluids and/or medication. However. any time the body's normal barriers (e.g. the skin) are broken (e.g. by placing a catheter through the skin into a vein), there is an increased risk of infection. Catheter site complications can range from mild inflammation of the skin around the catheter, to serious infection causing thrombosis (blockage) of the vein and abscesses.

Several routine practices are used to help reduce to risk of complications from catheters. Typically, a small (~2 cm X 2 cm) area of skin over the vein is shaved or clipped. Next, the area is usually cleaned with antibacterial soap, and then wiped several times with alcohol and a disinfectant like chlorhexidine or povidone iodine. This is done to help reduce the number of bacteria on the skin at the site where the catheter is placed so the site is less likely to become infected.

At some facilities, clipping  the neck before the catheter is places is often not performed. Also, sometimes owners request that the site not be clipped because they don't want it to be apparent that their horse needed a catheter because it was sick or had surgery. I've always been very reluctant not to clip, although there hasn't been a lot of objective evidence proving that clipping is important.

A recent study published in the Veterinary Record (Geraghty et al. 2009) has provided some information about the effects of catheter site preparation. In this study, researchers looked at different skin preparation methods. They cultured swabs from the skin before and after different procedures. 

The most important findings were:

  • Clipping and shaving had a significant effect on bacterial numbers, even before disinfection. Disinfection resulted in essentially eliminating all detectable bacteria, whether the haircoat was clipped, shaved or left intact.
  • Clipping, shaving or leaving the haircoat intact were equally effective if a disinfectant was used afterwards. However, clipping or shaving were still preferred over leaving the haircoat intact. (Clipping or shaving both reduced initial bacterial numbers, and this might be important in situations where the haircoat is highly contaminated or dirty).
  • Clipping and shaving may be useful for reasons other than disinfecting the catheter site. They can make the vein easier to see, which may make it easier to place the catheter. Difficulty placing the catheter and repeated attempts to place the catheter can cause  additional tissue damage and most likely increase the risk of complications. Leaving the haircoat intact also may increase the risk of introducing foreign material (e.g. hair, dirt) into the body when the catheter is placed.
  • Since it was possible to effectively eliminate detectable numbers of bacteria from an unclipped haircoat with proper disinfection, placing a catheter without clipping is acceptable "when rapid intravenous catheterisation is necessary as a clinical priority."  To me, this means that it's okay to place a catheter without clipping if the time needed for clipping might make a major difference to the chances of the patient surviving. This is not the case in situations where people just want to avoid evidence that their horse has had a catheter. While the true risk of not clipping is unknown, it makes no sense to not clip when there is no real downside (other than aesthetics) and there is a logical possibility that it could decrease the risk of complications.
  • Chlorhexidine and povidone iodine were equally effective disinfectants. However, chlorhexidine has been shown to be more effective in people at preventing infections. It has a broader spectrum of activity against microorganisms, and is less inhibited by dirt and debris. The small size of the study might have limited the ability to detect a difference between the two products. Since there is no real reason to use povidone iodine over chlorhexidine, and chlorhexiinde has been shown to be better in people, it makes sense to use it in horses.

This study provides some very useful information. Because it looked at bacterial numbers on the skin, not complications/infections per se, we have to be careful not to over-interpret the results. This study (as usual for studies like this) only assessed the transient bacterial population on the skin, not the resident population. The resident population is deeper in the skin and harder to kill. So, even though no bacteria were found after certain procedures, we can't assume the area was truly sterile. Nonetheless, the transient bacteria tend to cause most of the problems, so this type of study is still useful.

Intravenous catheterization is a very important tool in equine medicine. While there are always risks, the benefits of having a catheter in place almost always greatly outweigh any risks. Careful preparation of the catheter site, good catheter placement technique and close monitoring of the catheter site help reduce the risk of complications.

Susceptibility of Lawsonia to Antibiotics

Lawsonia intracellularis is an important cause of disease in weanling foals, causing a disease called proliferative enteropathy. Antibiotics, mainly erythromycin,are usually used as part of the treatment for this condition. However, little is known about the true antibiotic susceptibility of this bacterium. The problem is that Lawsonia does not grow in culture plates in a lab like most other bacteria with which we deal - it can only grow in cells, which makes it very difficult to test for  antibiotic resistance.  Antibiotic therapy for this disease is therefore chosen based on basic knowledge about the bacterium and anecdotal information about how animals respond to treatment.  Obviously, this is not the ideal situation.

A little more information is now available on this topic. In a recent edition of Veterinary Microbiology, researchers from the University of Minnesota published a study of the activity of different antibiotics on L. intracellularis. The study, by Suphot Wattanaphansak and colleagues, only involved 10 isolates of the bacterium, all of which were from pigs. (Lawsonia infection is an even bigger problem in pigs than it is in horses).  They only tested with a few drugs that are used in horses, and they used an unvalidated (but reasonable) test, so it's very important to be careful (and conservative) when extrapolating the results to Lawsonia in horses. However, they did have some potentially equine-relevant results. One important finding was that there was variation in susceptibility to some drugs between isolates, meaning some strains were susceptible and some were resistant to certain drugs. Although this isn't really surprising, it emphasizes the fact that the same treatment may not be suitable for all cases - antibiotic resistance can be present, and if it is it's critical to identify and address the issue.  This particular study showed that tetracycline, a drug sometimes used in horses, had "intermediate activity" against Lawsonia... not great, but not horrible. Unfortunately, they didn't test erythromcyin or chloramphenicol, which are two drugs more commonly used to treat Lawsonia in foals.

This study doesn't tell us much more about how to treat horses with this infection, but it does emphasize the need to test equine strains against drugs used in horses to see if we are doing things right, whether resistance to important equine drugs is present, and to determine if there are better and faster ways to detect resistance.

More information about Lawsonia intracellularis can be found on the equIDblog Resources page and in our archives.

Urinary Tract Infections in Horses

Unlike dogs, which commonly have urinary tract disease, urinary tract infections (UTIs) are very uncommon in horses, but they can occur.

  • Foals less than a month old are at risk of developing upper urinary tract infections (affecting the kidneys themselves) secondary to bloodstream infections (septicemia).
  • Critically ill foals that are given broad spectrum antibiotics to treat serious bacterial infections can (rarely) develop fungal infection of the lower urinary tract (affecting the urethra and bladder) caused by Candida sp. These infections can also “ascend” the urinary tract, eventually traveling from the bladder up the ureters to the kidneys as well.
  • UTIs in adults are very uncommon, but when they occur they usually affect the lower urinary tract. Mares are slightly more likely to develop UTIs than geldings and stallions because they have a much shorter urethra, so it’s easier for bacteria to reach the bladder (they don’t have as far to go!).

Upper UTIs are very serious because the kidneys themselves are affected (nephritis), which can ultimately lead to kidney failure. Animals with this kind of infection are typically very sick, depressed, and can have a fever. In general, lower UTIs are not as serious, but they do make it very uncomfortable for the animal to urinate (the same is true for people!). However, lower UTIs that result in or are the result of stones in the urinary tract can be life threatening if one of the stones becomes lodged in the urethra, preventing the horse from urinating altogether (this is similar to the situation with “blocked cats,” which occurs relatively commonly). Most of these infections are caused by the same common types of bacteriaE. coli, Proteus mirabilis, Klebsiella sp., Staphylococcus sp., Enterobacter sp., Corynebacterium sp., Pseudomonas aeruginosa – but sometimes fungi can be involved (such as Candida sp.) and there are also some parasites which rarely can affect the urinary tract of horses.

The important thing to remember, though, is that these infections are uncommon in general, and they are very rare in otherwise healthy horses. So if a horse has signs of a UTI (e.g. difficulty urinating, unusual behaviour when urinating, frequenting “squirting” without emptying the bladder completely, scalded skin from urine dribbling, blood in urine), there is often something else wrong. An important predisposing factor is bladder paralysis, when a horse loses control of the muscles in the bladder wall that make the bladder contract. When the bladder can’t contract, it never empties, and the urine just sits there, allowing bacteria to grow and sometimes even allowing infection to travel up to the kidneys (i.e. ascending infection). Bladder paralysis can result from diseases or certain toxins affecting the central nervous system (e.g. equine herpesvirus type 1 (EHV-1), Sorghum spp. toxicosis). In horses, it is often difficult to determine if stones (uroliths) are the cause of a UTI or the result of the UTI.

As in any other animal, the best way to definitively diagnose a UTI is to culture bacteria from a urine sample that has been collected as aseptically as possible (usually by passing a catheter, as in the photo above). It’s important to collect a urine sample for culture before starting any antibiotic treatment. If you suspect your horse may have a UTI, it should be examined by your veterinarian as soon as possible.

Infections in Neonatal Foals

Infectious diseases are a major problem in young foals. Diseases, including diarrhea, pneumonia, meningitis, umbilical infections and joint infections can range from mild to rapidly fatal.  Even in foals that survive the initial infection, these conditions can sometimes result in permanent problems. A study published in a recent edition of the Equine Veterinary Journal (Wohlfender et al, 2009) looked at infectious diseases in the first 30 days of life in 1031 foals on 36 breeding farms in the Newmarket, UK area. This very large study provided some interesting information:

  • The overall incidence of infectious diseases was 8.3%, meaning around 1 in 12 foals developed some type of infectious disease during the first 30 days of life.
  • The most common infectious problem was diarrhea, which occurred in 5.9% of foals. Foals with diarrhea that didn't have other signs of illness were not included.  This is because most young foals with diarrhea and no other abnormalities likely have foal heat diarrhea, which is not an infectious disease.
  • Umbilical infections developed in 0.7% of foals.
  • Joint infections occurred in 0.4% of foals.
  • Bone infections occurred in 0.2% of foals.
  • Respiratory infections were uncommon, occurring in only 0.6% of foals.
  • There was no difference in the incidence of infections in foals that were treated routinely with antibiotics to "prevent" infections. Whether or not this practice is effective has been a controversial issue for a long time.  The best way to answer the question would be to have a study that randomly assigned foals to receive antibiotics or no antibiotics, and then monitored both groups for infections.  This study didn't do that, so there could be some "bias" to this particular finding, but the results are still useful. I don't find the results surprising - there has never been good evidence that antibiotics are routinely needed in newborn foals. This study may help control routine (and in my opinion unnecessary) antibiotic treatment in healthy newborn foals.

This study provided more information confirming that infectious diseases are a significant problem in foals, and that measures are needed to reduce the incidence of infections. It also provided evidence that routine antibiotic use is NOT an effective means of doing this. While there is a lack of good, objective evidence, routine hygiene, sound management and every-day infection control practices are probably critical factors for infectious disease prevention in these young animals.

Treating Abscesses in Horses

Abscesses are common problems in horses. They can range from easy-to-identify-and-treat foot abscesses to abscesses that are very difficult to diagnose and even harder to treat (e.g. brain abscesses, abdominal abscesses).

An abscess is an accumulation of pusPus is basically dead bacteria, partly broken-down cellular debris, and white blood cells. Sometimes there can be a thick capsule of tough, fibrous tissue surrounding an abscess, particularly if it has been there for a long time (see picture left). Most abscesses occur as the result of a local infection that the body was able to contain (at least partly), but could not eliminate altogether, and that didn't "drain" naturally to an open space (e.g. outside the body). Over time, some abscesses may continue to grow and become quite large, which can cause many different problems depending on where the abscess is. "Sterile" abscesses can also occur, but are much less common - these lesions are not associated with a bacterial infection, but may occur following a severe trauma or in association with certain tumours.

Because abscesses are usually caused by an infection, it's logical for people to think about using antibiotics to treat them. However, antibiotics are not very effective at treating abscesses because:

  • Abscesses have very little blood supply. A good blood supply is needed to deliver antibiotics to an infected site.
  • Many antibiotics do not penetrate into abscesses well because of the tough capsule and the type of fluid within the abscess. Some do a better job than others, but none are great.
  • Even if an antibiotic is able to penetrate an abscess, it may be only minimally effective due to the amount of pus and bacteria that are usually present.

 So, if we can't use antibiotics effectively, what do we do? The most effective treatment for any abscess is incision and drainageThis involves lancing the abscess to remove as much of the infected pus as possible. It also allows for local treatment such as flushing with antiseptics.  Antibiotics should only be used when there is no way to drain (lance) an abscess, such as with lung abscesses.

If an abscess can be lanced, it should be, with very few exceptions. That may involve paring out a foot abscess, lancing a strangles-infected lymph node, or opening a large abscess from a wound. Lancing of abscesses should always be done by a veterinarian. While it may be tempting to "pop" an obvious abscess yourself, there may be important blood vessels or nerves in the area that need to be avoided. The abscess that you see may be only one part of the problem, and other diagnostic tests or treatments may be needed. A veterinarian can pick the best part of the abscess to lance, to ensure the best possible drainage, and a veterinarian can provide proper sedation and local anesthesia for the horse during the procedure. An ill-placed stab at an abscess can make things a lot worse.

Photo: A large abdominal abscess from a horse that was drained and opened to show the very thick outer fibrous capsule, and many thinner, internal loculi. (M. Anderson)

Piroplasmosis Quarantine Finally Over in Florida

In August 2008, there was an outbreak of equine piroplasmosis in the state of Florida.  The index case was a gelding that was euthanized following a three-week illness.  The diagnosis was made based on testing of samples collected at necropsy.  The US has been considered free of this reportable foreign animal disease since 1988, so the discovery lead to automatic notification of the authorities, who started an investigation and implemented quarantine of numerous facilities.  During this outbreak, 20 horses on 7 farms were identified as infected. A total of 25 farms were quarantined.

Representatives from the state of Florida announced recently that the last farms quarantined during the August 2008 outbreak of equine piroplasmosis have now been released. All horses are now free of the parasite. Florida (and the US as a whole) is once again considered free of equine piroplasmosis.

Equine piroplasmosis is a blood-borne disease that is caused by the parasites Babesia equi and Babesia caballi.  Infected horses may develop mild disease characterized by weakness and loss  of appetite. In more serious cases, horses may develop fever, anemia, jaundice (yellow gums and eyes), a swollen belly and respiratory problems. Other signs that are sometimes present include neurological abnormalities, red urine (secondary to destruction of red blood cells) and death. In contrast, many infected horses may no signs of illness whatsoever. Horses that recover may carry the parasite for a long period of time, and these horses can be a source of infection for other horses if there are ticks in the area capable of transmitting the parasite (not all species of ticks can transmit the disease). This can lead to the need for long quarantines. Horses that become persistent carriers of piroplasmosis need to  be quarantined for life, euthanized or sent to a country where the disease is endemic.

How this outbreak started and spread is unclear. No infected ticks were ever identified. A recent update concluded that "Evidence uncovered during the investigation indicated that transmission of the [equine piroplasmosis] organism occurred due to management practices and not by natural transmission which occurs via ticks." I'd love to know exactly what they found and what specific "management factors" were the problem.  Blood-borne pathogens can be transmitted in some cases on equipment that is contaminated with blood, such as reused needles and dental equipment.

A good fact sheet on equine piroplasmosis is available from the US Animal and Plant Health Inspection Service (APHIS).

Photo: Red blood cells infected with Babesia spp. (source: CDC Public Health Image Library, ID#5943)

Bug of the Month: Actinobacillus equuli

Actinobacillus equuli is a common Gram-negative (i.e. looks red/pink with Gram stain) bacterium, the natural host of which is (not surprisingly, based on the name "equuli",) the horse. This organism can be found quite commonly in or on healthy horses, for example, as part of the normal bacterial flora of the upper respiratory tract.  However, A. equuli can cause disease in certain situations.

Horses that most often become infected and sick from A. equuli are foals. This bacterium can cause a wide range of infections in these young animals, including septicemia (bloodstream infection), meningitis, pneumonia, omphalophlebitis (umbilical infection) and septic arthritis (joint infection). Foals can get infected from contact with other horses or even their own mares because the bacterium is so common.  However, it typically only becomes a problem in foals that do not receive adequate colostrum immediately after birth, or that do not receive proper umbilical care.

Disease in adult horses due to A. equuli is uncommon. As in foals, the bacterium can cause a variety of infections in adults, including pneumonia, wound infections and guttural pouch infections. These infections are usually opportunistic - they occur following another problem that affects the animal's normal defense mechanisms, such as viral pneumonia or lacerations. Primary infections with A. equuli, including peritonitis, pleuritis and septicemia, can occur in healthy adult horses, but are very rare.

Infection with A. equuli is diagnosed by culturing the bacterium from the infected body site.  Culture also permits identification of what antibiotics may or may not be effective for treating the infection.  Actinobacillus equuli is usually susceptible to a variety of routinely-used antibiotics.

Mare Mastitis

Mastitis (inflammation of the udder) is a relatively uncommon problem in mares. Some of the likely reasons that mares develop mastitis much less commonly than other animals (such as cows) is that they have comparatively small teats, and the udder, even when engorged, remains well tucked up between the hind legs. This decreases the risk that the teats will be traumatized or soiled compared to the large, low-hanging teats of a cow. Also, frequent nursing by foals (normally several times an hour) keeps the udder relatively empty and helps prevent build up of bacteria in and around the teat. The fact that people don’t frequently touch a mare’s teats and udder also helps prevent contamination with bacteria from our hands or from other sources that may be transmitted indirectly on dirty hands.

When a mare does develop mastitis, it can cause quite a significant problem, for both the mare and her foal. For one thing, mastitis is usually very painful! Even the most well behaved, tolerant mare will usually object to foal trying to nurse. Usually the mare will simply move away from the foal, but very persistent foals may also get kicked. Beyond this, problems for the foal are two-fold:

  • The foal will not receive adequate nutrition and will quickly run down its body’s own meagre reserves, thus making it more prone to infections and other complications.
  • If the foal is able to nurse at all from the inflamed part of the udder, it is drinking milk that is often heavily laden with the bacteria causing the infection, which represents an additional challenge to its body’s defenses and immature intestinal flora.

The infection in the mare is usually bacterial in nature, and the culprits tend to be opportunistic bacteria that can be found around any horse, especially Streptococcus zooepidemicus, but also Staphylococcus, Actinobacillus, Pseudomonas, Klebsiella and Escherichia coli. In mild cases the infection will be limited to the udder. In severe cases, and especially with Gram-negative infections, mares can develop systemic illness due to endotoxemia or septicemia, which can lead to complications like colitis and laminitis (founder). Mastitis may occur in only one half (gland) of the udder, or in both parts of the udder at the same time.

Mastitis is diagnosed by culturing bacteria from the milk. It’s very important to take a culture sample before beginning treatment in case the bacteria involved aren’t sensitive to the initial antibiotic treatment. Mares are usually also treated with an anti-inflammatory to help keep them more comfortable. However, the most important component of therapy is stripping the udder (i.e. milking the mare out) as often as possible to remove the bacteria and all the inflammatory debris. Hot packing the udder helps to promote drainage, and hand walking helps decrease edema and swelling around the udder.

Most mares recover from mastitis in 5-7 days with proper treatment, but it’s important to initiate treatment as early as possible, before the infection becomes severe. Signs to look for that may indicate a lactating mare is developing mastitis include:

  • The mare being persistently reluctant to allow the foal to nurse
  • Abnormal swelling of the udder, particularly if it is hot and painful on palpation
  • Abnormal-looking milk (often watery with white flecks/chunckies) from one or both teats
  • Sudden development of ventral edema around the udder
  • Reluctance to walk or abnormal gait in the hind limbs
  • Non-specific signs like fever, lack of appetite, and depression

If you suspect your mare may be developing mastitis, she should be examined by a veterinarian as soon as possible. It is also very important to monitor the foal in these situations – if the foal is not able to get sufficient milk from the mare, it may need to be supplemented by bottle or bucket feeding until the mare recovers.

  • Avoid touching the teats and udder of normal mares whenever possible in order to decrease the risk of contaminating the teats with bacteria from your hands.
  • If you need to touch/check the udder, wash your hand thoroughly first with soap and water (preferably warm water - most mares appreciate that!).

Vaccinating Foals

While it's a little early in the foaling season, it never hurts to think about vaccination plans for foals. Vaccination is an important part of the disease prevention program - but it's only one part... general infection control practices are as, or often even more, important. Vaccination of foals is not the same as vaccination of adult horses. Foals may be more or less susceptible to certain infections. They may have greater or lower risks of exposure, and they may respond differently to vaccines.

There is no standard vaccination program for foals. Specific vaccination programs need to be designed for each horse on each farm based on risk of exposure and infection, cost and many other factors. Your veterinarian can help you determine what your animals should be vaccinated against. When it comes to foals, here are some important things to considered:

  • Foals typically need to be vaccinated several times to get the desired immune response. There are at least a couple of reasons for this. First, if a foal has high antibody levels from colostrum, it may not respond properly to the vaccine until those antibodies are used up. These antibody levels drop over time, but the rate and timing of the drop is different for each foal. Vaccinating the foal several times (at appropriate intervals) helps reduce the risk of vaccine failure from colostral antibodies. Second, foals are born with a fully functional immune system, but the immune response to an infectious agent or vaccine is slow and low the first time an individual encounters it. The first dose of vaccine may produce some response, but a much higher response is generated with boosters.  This is actually true of adult horses as well.
  • Over-vaccination can be a problem. Vaccination too early and/or too frequently could actually result in a decreased immune response or complete vaccine failure, a phenomenon known as immune tolerance. So, giving many doses of vaccine starting at a very young age may actually be counter-productive. This is probably a bigger concern with influenza vaccination compared to other vaccines.

Talk to your veterinarian about vaccination. Over-, under- and improper vaccination can lead to increased disease risks, plus wasted time and money.

Guidelines for vaccination of foals have been developed by the American Association of Equine Practitioners. These are also available from the link on the equIDblog Resources page.

Management of Chronic Grass Sickness

Equine dysautonomia, or "grass sickness", is a very important disease in some areas of the world, particularly the United Kingdom and some parts of South America (where it's called mal seco). The cause of the disease is still unclear, but there is increasing evidence implicating the bacterium Clostridium botulinum, which produces the toxin that causes botulism. There are several forms of grass sickness, one of which is the chronic form. This form of grass sickness is potentially survivable (unlike the other forms which are invariably fatal), although treatment can be long and difficult. To help guide people managing horses with chronic grass sickness, a free-access online booklet has been released by World Horse Welfare and the Royal (Dick) School of Veterinary Studies. It is a comprehensive booklet that provides a wealth of information about this disease, with an emphasis on how to care for affected horses. The document can be downloaded here or by visiting the World Horse Welfare website.

Bug of the Month: Streptococcus zooepidemicus

Streptococcus zooepidemicus (technically Streptococcus equi subspecies zooepidemicus), commonly called Strep zoo, is an important bacterium in equine medicine. It is one of the most common bacteria isolated from infections in horses. Like other streptococci, S. zooepidemicus is a Gram positive coccus, meaning that it stains purple with Gram stain and has a ball shape (coccus). Streptococci tend to stick together in chains (see picture right) which are often described as "string of pearls."

Streptococcus zooepidemicus can be found in the upper respiratory tract of healthy horses. In most instances, it lives there without causing any problems.  Strep zoo is considered an opportunistic pathogen - a bacterium that can live in harmony with its host, but can also cause disease in certain situations. Strep zoo  can cause a variety of infections, including most commonly pneumonia, lung abscesses and guttural pouch infections. More severe conditions, like bloodstream infections, joint infections and meningitis, can also occur, but are uncommon and most often affect neonatal foals.

Diagnosis of disease caused by S. zooepidemicus requires culture of appropriate samples collected from the horse. Care should be taken when interpreting cultures of samples collected from the nasal passages or upper respiratory tract, since S. zooepidemicus is often present there even in normal animals, so finding it does not necessarily mean it is causing disease.

Unlike some bacteria that cause infections in horses, S. zooepidemicus tends to be susceptible to numerous antibiotics, including penicillin and trimethoprim-sulfa. However, since some strains can be resistant to various drugs, culturing the bacterium and testing its susceptibility to antibiotics in the lab is still required to determine the optimum treatment, .

Strep zoo is of minimal risk to people. Human infections caused by this bacterium have been reported but they are considered very rare.

Commercial Probiotics: Let's Make Up A Name!

I just got back from the North American Veterinary Conference in Orlando, FL. One of the talks I gave at the conference was about probiotics, during which I spent a fair bit of time talking about various concerns with commercial probiotics. Part of this relates to a study we did in 2002 looking at probiotic product labels. We found that the label descriptions on most products were unacceptable, including many that misspelled the contents and others that claimed to contain organisms that do not exist.

It was quite funny when, immediately after that talk, I went to the tradeshow and saw a booth for a new probiotic product that is apparently quite popular in Italy. It claimed to contain four probiotic organisms, including Lactobacillus sporogenes. Unfortunately, Lactobacillus sporogenes does not exist!  There are no spore forming Lactobacillus species, and this particular bug was renamed Bacillus coagulans in 1939!  Some people market it as a Lactobacillus, presumably because lactobacilli are more widely thought of as potentially beneficial organisms. The person at this product's booth didn't particularly like the fact that I said the organism doesn't exist, but couldn't come up with a rebutal. The product also had no efficacy data or in vitro data suggesting that any of the organisms it contained had the potential to be beneficial, as is the case with most of these products.

I continue to be disappointed by the lack of good research behind most commercial veterinary probiotics. Hopefully, someday, companies will start spending some money on research instead of just marketing. There is more promise with some newer small animal probiotics, as it appears more research is being done, but that same degree of effort doesn't appear to be there for equine probiotics.

More information on probiotics can be found on the equIDblog Resources page.

Sickbed Musings: Horses, Vomiting and Clostridium difficile

There's something ironic about traveling to a conference to speak about infectious diseases and coming down with food poisoning.  As I lay in bed in Orlando in a relatively non-functional state Friday, I had lots of time to contemplate the good and bad points of vomiting. As much as it is unpleasant, the ability to vomit is useful, and it is something horses lack. As one of the many anatomical quirks that horses possess, horses are essentially unable to vomit, regardless of what in happening farther down the intestinal tract. This is serious problem, because unlike people who can relieve the pressure of a distended stomach by vomiting, the only thing a horse's stomach can do is get bigger and bigger until it finally bursts. Stomach rupture is fatal and kills numerous horses every year - it causes massive infection in the abdomen (peritonitis) due to the spillage of the bacteria-laden intestinal contents.

Stomach rupture typically occurs secondary to obstruction of the small intestine, which usually requires surgery to fix. It can also result from a condition called duodenitis/proximal jejunitis or anterior enteritis. This disease has been an enigma for years, with no clearly identifiable cause. Recently, the bacterium Clostridium difficile was implicated as the cause of this syndrome, at least in Ontario.  This bacterium was found commonly in stomach fluid from horses with anterior enteritis, but not in that of horses with gastric distension from other causes.  More study is needed to determine if these results are repeatable over time and in other regions, but it may be an important insight into this uncommon but potentially life-threatening condition.

More information about Clostridium difficile can be found on the equIDblog Resources section.

Canadian Update on CEM Outbreak

The investigation of the outbreak of contagious equine metritis (CEM) in Kentucky last month continues throughout many states in the USA, and has also spilled over into Canada.  At this time, four farms in Alberta and six farms in Ontario remain under quarantine.  These farms either received frozen semen from one of the three infected Kentucky stallions in the spring of 2008, or have mares that were shipped to Kentucky for artificial insemination with semen from one of these stallions.  So far, no confirmed cases of infection have been reported on any of these farms.

The Canadian Food Inspection Agency (CFIA) is responsible for the investigation of reportable animal diseases in Canada, including the current CEM investigation.  Representatives of the CFIA will present an update on the situation at the Breeds & Industry Delegate Assembly at the Equine Canada Convention in Ottawa on February 7.

Any horse in Canada suspected of being affected by CEM must be reported to the CFIA.  The most apparent, classic sign of CEM in mares is a thick, purulent (pus) vaginal discharge after breeding, without systemic illness (i.e. no fever or depression).  However, some mares and almost all stallions can carry the causative organism, Taylorella equigenitalis, without any obvious clinical signs of infection, which is why the disease can be difficult to control if people aren't careful.  Taylorella equigenitalis does not affect people.

The CFIA has made several recommendations to Canadian horse owners to try to prevent the spread of this disease into Canada, and to prevent its spread should it be found here at any time:

  • Until the outbreak investigation in the USA is complete and the disease is contained, use extreme caution or simply refrain from importing breeding animals, semen, embryos etc. from the USA.
  • Emphasize strict hygiene measures when handling breeding animals.  Wear disposable gloves when washing or otherwise coming into contact with a horse's tail or genitalia.  Change gloves (and wash your hands!) before handling another horse.
  • Use separate equipment (e.g. buckets, sponges, tail wraps) for each horse.  Any equipment that must be shared should be thoroughly cleaned and then disinfected between horses.  Taylorella equigenitalis is susceptible to most common disinfectants, including bleach.

Potomac Horse Fever

Potomac Horse Fever (PHF), a disease named for the river near Washington DC where it was first identified, is caused by Neoricketsia risticii.  The technical name for PHF is equine monocytic ehrlichiosis (EME).  This causative organism is normally found in a trematode parasite with a somewhat complex life cycle involving bats, snails and certain aquatic insects. It is therefore associated with swampy/marshy areas in certain regions.  Horses are aberrant hosts of N. risticii, in which infections can cause a range of disease from very severe colitis and laminitis to mild non-specific illness.  The organism is typically very sensitive to treatment with tetracyclines, but the disease can progress very quickly, so treatment early in the course of disease (if PHF is strongly suspected) is crucial.

In general, PHF is an uncommon disease in horses, but there are certain areas of Ontario where cases occur with some regularity. For example, cases have been reported in Eastern Ontario (Brighton to Kemptville), Keswick area and Ancaster. The incidence is usually highest in late summer. Transmission is normally associated with horses grazing pastures containing infected flies (caddisflies).

Although vaccines for PHF are available and appear to be safe, their efficacy is very questionable. At least in part, this may be due to the fact that multiple strains of N. risticii occur naturally, but only a single strain (from the 1980s) is included in the vaccines.  Vaccination is not considered necessary for horses in areas where the disease has not been previously reported. Given the peak incidence of the disease in late summer and the nature of the vector, if someone wants to vaccinate, they should do so in late spring. Preventing access of horses to areas near swamps and other insect breeding grounds may be as (or more) effective than vaccination alone.

Photo Credit: Buck Cash, Great Falls, Potomac River, VA

 

Tetanus Antitoxin in Foals

I was reading a fact sheet on foal care today that was available online. It was one of those sources that gives a mixture of good, mediocre and bad information. One thing that I didn't like was a reference to tetanus antitoxin administration being a common practice in newborn foals. That might have been the case a couple of decades ago, but it is certainly not recommended now.

Tetanus is certainly a serious problem in horses, and foals are at risk for exposure because the bacterium that causes the disease, Clostridium tetani, is widespread in the environment. Good measures need to be in place to reduce the risk of foals developing tetanus. The most important steps are:

  • Ensure the mare is properly vaccinated for tetanus prior to foaling. This includes a booster that should be given approximately 30 days before foaling.
  • Ensure that the foal gets enough good quality colostrum (see the information sheet on the Resources page for more details about colostrum for foals).
  • Treat the umbilicus properly after birth (for more information, see our previous post entitled "Starting Out Right - Foal Umbilical Care").

If these things are done, there is no need to give tetanus antitoxin to a newborn foal. In fact, it's not only unnecessary, it's a potential health risk. Tetanus antitoxin neutralizes tetanus toxins that are in bloodstream. It used to be widely used in horses of all ages if they were wounded. However, administration of tetanus antitoxin has been associated with a disease called Theiler's Disease or serum hepatitis. While rare, this is a severe and almost always fatal liver disease.

Tetanus antitoxin is only potentially indicated in foals whose mares were not vaccinated against tetanus (or their vaccination status is unknown) and possibly in foals that did not receive adequate colostrum.

More information on tetanus, colostrum and vaccination recommendations can be found on the  equIDblog Resources page.

Contagious Equine Metritis - USA Outbreak

In December 2008, an outbreak of contagious equine metritis (CEM) was reported in Kentucky. The index case was a healthy stallion that underwent routine testing for exportation of semen and the causative agent, Taylorella equigenitalis, was isolated by cutlure on December 15. By the end of the month, three more stallions on the same farm were also identified as infected. At the moment, it is estimated that there are 28 horses in Kentucky and another 156 horses outside Kentucky that have been exposed. Another 250 horses are being traced across 27 states. The last outbreak of CEM in the USA was in 1979 in Missouri, although a few sporadic cases have been identified in the country in the last three decades. It is a reportable disease in both the USA and Canada, and there are strict import (and export) regulations for horses entering the USA and Canada from CEM-positive countries.

CEM is a venereal disease of horses. It is spread by stallions who can carry the bacteria on their external genitalia without any noticeable clinical signs, as well as by infected mares, during breeding. The bacterium can also be transmitted through artificial insemination, particularly on contaminated equipment. The diseases causes temporary infertility in mares. Typically mares do not conceive when they are first infected, but if a mare does become pregnant she may abort, or the foal may be born a carrier. Some mares develop a heavy purulent vaginal discharge (pus) for up to two weeks after breeding, but others may only have a shortened heat cycle. Some mares will clear the infection on their own, but in some cases a mare may become a chronic carrier of T. equigenitalis and transmit the bacteria to stallions during breeding. The organism does not cause any clinical signs in stallions, so the only way to detect the bacteria in males is to culture it. However, T. equigenitalis is notoriously difficult to grow in a laboratory, so false-negative results can occur. Therefore adjunctive tests are also used, and additional testing methodologies (including molecular techniques such as PCR) are being developed for the detection and study of this organism.

The good news is that infected horses can be treated, and T. equigenitalis is typically susceptible to most common antibiotics. Local treatment and repeated flushing of the external genitalia of infected horses is also part of the treatment regimen. Compared to other diseases, the spread of CEM is also relatively easy to control, because it is only transmitted by breeding (either live cover or artificial insemination). The major problem is that this disease is also one that can easily “fly under the radar” for some time before it is discovered, because carrier animals show no clinical signs of infection, and stallions in particular, as in this case, may expose many mares before the bacterium is discovered. Hopefully this outbreak will be brought rapidly under control so that the equine industry in the USA can avoid devastating economic loses due to the presence of this disease.

Rabies Vaccination in Horses: Core Issues

This post originally appeared on the Worm & Germs Blog site on December 12, 2008.

In 2008, the American Association of Equine Practitioners (AAEP) published updated vaccination guidelines for horses.  One of the changes from the previous set of guidelines was the inclusion of rabies as a core vaccine (meaning every horse should receive it).  There was lots of discussion about this at the recent AAEP Annual Convention in San Diego, CA.

Some veterinarians don't like the idea of vaccinating every horse against rabies.  Just like veterinarians and owners of dogs and cats who are concerned about over-vaccination in these species, the same concerns exist in equine medicine.  Equine rabies vaccines are not approved for use every three years like some canine and feline vaccines, so they still need to be given every year until someone can determine for how long a vaccinated horse is protected from infection.   Furthermore, there has never been (to my knowledge) a case of human rabies due to transmission from a horse.  These are all valid points, but there are also a lot of reasons why including rabies as a core vaccine for horses is very good idea:

  • Rabies is a very deadly disease, in both animals and people.  To some owners, their horse is every bit a part of their family as any dog or cat could be.  To other owners, their horses represent a great investment, and part of their livelihood.  Even if the risk of disease in horses is low, protecting them is safe and easy, so it just makes sense.  As the saying goes, an ounce of prevention is worth a pound of cure, but when there is no cure and prevention is so simple... you do the math.
  • Rabies vaccination is extremely effective in horses, producing an excellent immune response even with a single dose.  It does not require complex adjuvants that some other vaccines need to stimulate the immune system, which also makes it less likely to cause an abnormal vaccine reaction.
  • Rabies is not a seasonal disease like many of the respiratory viruses or insect-borne diseases (e.g. West Nile) for which horses are also typically vaccinated.  Rabies boosters only need to be given once a year, so this can be done during a time of year when no other vaccines are required, if there are concerns about giving too many vaccines at once.
  • Horses live outside and in barns.  Most are far less supervised than dogs and cats, but even these animals are at risk of rabies exposure.  A rabid animal could easily be "brave" enough to attack a horse, even though it normally wouldn't.  Bats can also easily get into and out of many barns - you may never know one was there, and finding a bite mark from a bat on a horse would be like looking for a needle in a haystack, but that's all it takes to transmit the virus.  So it makes sense to give your horse added protection by vaccinating it.
  • Rabies in horses may not look like rabies at first.  One of the most common early signs is actually colic.  A rabid horse that looks like a colic may expose the people who are trying to look after it before they realize what the horse has.  In other horses the signs may be recognized too late, like the rabid horse that was found at the Missouri State Fair earlier this year, that resulted in exposure of many people.
  • While rabies transmission from horses to people has not been documented, rabid horses have killed people, particularly horses that develop the "furious" form of rabies, which can cause them to become very violent.

For more information on rabies, see our rabies archive or the information sheet available on the equIDblog Resources page.

EPM in Horses - Eliminate Possum Muck

The Frank J. Milne State-of-the Art Lecture at the recent 2008 AAEP Conference was delivered by Dr. Stephen Reed of Rood and Riddle Equine Hospital in Kentucky. The lecture was entitled “Neurology is Not a Euphamism for Necropsy: A Review of Selected Neurological Diseases Affecting Horses.” The three major diseases that were reviewed were cervical stenotic myelopathy (Wobbler syndrome), equine herpesvirus (EHV-1) myeloencephalopathy, and equine protozoal myeloencephalitis (EPM).

EPM was first identified in horses in the 1970s, but it took more than twenty years before the causative agent, the protozoal species Sarcocystis neurona, was identified. Shortly thereafter, the definitive host of S. neurona was identified as the opossum (specifically the Virgina opossum (Didelphis virginiana) in North America). In the opossum, the parasite lives and reproduces in the animal’s intestine without causing much damage, and oocysts are passed in the feces. Horses, on the other hand, are aberrant or “dead end” hosts – if they ingest the parasite from the opossum feces, they cannot spread it to other animals of any species. However, in a horse, the parasite can leave the intestine and migrate through the animal’s tissues,  ultimately reaches the brain and/or spinal cord. Damage to the nervous system caused by migration of the parasite. Although EPM has been identified in horses outside of the Americas, the vast majority of these cases are horses that were imported from or at one point traveled to the New World.

EPM commonly gets blamed for a lot of minor abnormalities in horses (mostly gait abnormalities), often without being properly diagnosed. Part of the problem is that exposure to the parasite in the New World is very common (>50% of horses in many areas), but definitive diagnosis of disease due to EPM can be very difficult. The classic sign of EPM is pronounced asymmetrical muscle wasting (note the profound muscle wasting of the left gluteal muscle in the picture) in different parts of the body. There are very few other diseases that result in this kind of condition, so a fairly confident diagnosis can be made in these cases. Horses with more subtle or less classic signs are more problematic.

  • If a blood test is positive for antibodies to S. neurona, it only means the horse has been exposed to the parasite, not that it has EPM.
  • If the blood test is negative it means the horse probably hasn’t been exposed to S. neurona, but there have been a few reports of EPM caused by protozoa from the Neospora genus as well, which throws another wrench in the works.
  • We used to think that the way to diagnose EPM was to test the horse’s cerebrospinal fluid (CSF) for antibodies. The theory was that antibodies from the bloodstream could not get into the CSF because of something called the blood-brain barrier, so any antibodies found in the CSF would have to have been produced in response to parasites in the central nervous system itself. Unfortunately, it’s since been discovered that some antibodies from the bloodstream can leak into the CSF, even in a normal horse. However, analysis of CSF can still provide other information in some neurological horses, so CSF taps are still performed in some of these animals.  It’s important to realize, though, that this procedure is also not without its risks.

The consensus statement on diagnosis of EPM published by the ACVIM in 2002 is available on the equIDblog Resources page. It is a few years old now, but still contains a lot of good information.

Watch for more information on EPM in an upcoming post!

Opossum photo credit: M. Anderson, 2007.
 

Are Wildlife A Source Of Lawsonia?

Lawsonia intracellularis is a bacterium that causes an intestinal disease called proliferative enteropathy in young horses. One of the reasons we still don't know a lot about this disease is that this bacterium can't be grown in a lab. One of the areas in which researchers are working to find more information is from where horses that become sick initially get Lawsonia...  Is it from healthy horses that are carriers? Does it circulate in the horse population, or is there an outside source? Does it come from pigs (pigs can also be affected by disease due to Lawsonia infection)?  Do other animal species such as wildlife carry it?

A recent study by researchers at the University of California (Davis), published in the Journal of Wildlife Diseases (2008 44:992-998), looked at the presence of Lawsonia in wildlife on horse farms. They found DNA from the bacterium in feces from jackrabbits, striped skunks, Virginian opossums, and coyotes, but not from feral cats, Brewer's Blackbirds, raccoons, or ground squirrels.

This study provides some interesting insights about Lawsonia in horses, and raises the possibility that wildlife could be a source of infection. However, it does not tell us how to prevent the disease or whether wildlife really are a relevant issue when it comes to infecting horses. We certainly do NOT recommend trying to eradicate wildlife in the vicinity of horse farms as a Lawsonia prevention tool. However, making horse barns (especially feed storage areas) less inviting to wildlife is a good idea - this is a sound standard infection control measure that everyone should consider, as it may reduce the risk of several infectious diseases (e.g. equine protozoal myeloencephalitis (EPM)).

More information on Lawsonia can be found on the equIDblog Resources page, and in our earlier post entitled "Lawsonia intracellularis - New Horizons".

Equine Infectious Anemia - Eradication From Ireland

Another presentation from the recent 2008 AAEP Convention, this one by Dr. Simon More, described the outbreak and successful eradication of equine infectious anemia (EIA) from Ireland in 2006.  A review of the outbreak itself, its investigation and management was also published in the most recent issue of the Equine Veterinary Journal (2008; 40(7):702-711).

Equine infectious anemia (EIA) (aka "swamp fever") is a very important disease in horses, and can also affect donkeys and mules.  This is the disease for which horses are given a Coggins test, which is required every six months for horses attending most shows and competitions, and for horses traveling internationally.  EIA is caused by a retrovirus, more specifically a lentivirus.  Other important viruses in this group include several immunodeficiency viruses such as HIV.  When the EIA virus infects a cell, it actually permanently incorporates its genes into cell's DNA, so affected animals are infected for life.  Horses go through repeated bouts of illness - the earliest episodes are usually the most severe.  Horses that survive the acute phase become persistent, often inapparent carriers, and serve as a reservoir for the virus.  The primary means of transmission is via transfer of blood from infected horses.  This can occur through blood-feeding insects such as horse flies and deer flies, or through use of blood-contaminated equipment such as dirty needles, surgical instruments and dental equipment.

Signs of EIA during acute episodes include fever, depression, lack of appetite, decreased red blood cell count (anemia) and decreased platelet count  (thrombocytopenia).  Lack of platelets can lead to petechial hemorrhages (tiny dots of blood, often first noticed on the gums or inside of the lips).  Episodes typically last 3-5 days, but weeks or even months may go by between episodes.  Illness may also be precipitated by stressful events or treatment with immunosuppressive drugs.  In some horses the episodes become more frequent, and signs of chronic EIA develop.  In addition to anemia and thrombocytopenia, these horses (often called "swampers") become very thin and may develop edema over their lower abdomens.  If the condition becomes very severe, a horse may have very pale or yellow mucous membranes (e.g. gums, around the eyes), and some may have nose bleeds or even neurological signs.

Prior to the outbreak of EIA in June 2006, Ireland was free of EIA, an advantageous status for the horses who lived there, as well as their owners.  Between June and December, 38 horses became infected with EIA.  It is believed that the outbreak started with four foals that were given hyperimmune plasma for prevention of Rhodococcus equi infection that had been illegally imported from Italy.  It is likely that the plasma was contaminated with the EIA virus, but unfortunately by the time the outbreak was identified there was no remaining plasma that could be tested.  Transmission of the virus was thought to have occured through mechanical transfer of blood during veterinary procedures and vector transmission via horse flies. Additionally, it was suspected that several mares may have been infected through close contact with foals.  Ongoing surveillance in Ireland has not identified any additional cases in the last two years, so it is believed that the disease has been eradicated.  Investigation and control of the outbreak took a lot of cooperation between many different organizations and individuals.  The study of the epidemiology was also very important in controling the disease, as not all of the cases followed the book!

Lawsonia intracellularis - New Horizons

On December 9, the Infectious Diseases session was held at the 2008 AAEP Conference in San Diego, CA. Topics presented covered several conditions, including MRSA, Clostridium difficile, equine infectious anemia (EIA), parasite resistance and deworming protocols, and Lawsonia intracellularis.

Lawsonia intracellularis is a bacterium that causes a disease called (among other things) proliferative enteropathy in foals and weanlings. More information about this disease is also available on the equIDblog Resources page. This disease is relatively new in the horse world, being first identified in the 1990s. It has traditionally been considered a disease of pigs, where it causes several syndromes of clinical disease, including one similar to that seen in foals. In pigs that carry the bacteria in their intestine, clinical disease is brought on by stressful events, and the same may be true in foals. The infection causes severe thickening of parts of the small intestine, and the inflammation causes loss of large amounts of protein from the bloodstream. Affected foals are usually very “unthrifty” and quiet/depressed, and many often develop edema (non-painful, cool swelling) along their abdomens or under their jaws.

In pigs, prevention of disease due to Lawsonia infection involves decreasing stressful events and vaccination with an oral vaccine. Dr. Nicola Pusterla of the University of California (Davis) presented the results of a study that was done recently looking at the effects of vaccination of foals against Lawsonia in three different ways – giving the vaccine orally, giving the vaccine orally after treating the foals for three days with a gastroprotectant (omeprazole), and giving the vaccine rectally. The reason for the last two groups was to decrease the risk of the vaccine being destroyed by the acidic environment of the normal stomach. Indeed, they found the antibody response to the vaccine in the bloodstream was better in these groups than in the untreated foals that were given the vaccine orally. Of the 12 foals that received the vaccine, all of them stayed healthy throughout the study period (42 days).

Although seeing some work on developing a vaccine for this disease is great, we are still a long way from being able to use vaccination to prevent disease in foals. While the animals in the study produced antibodies in the bloodstream, Lawsonia actually lives inside cells, where antibodies usually cannot get to them. Immunity against intracellular pathogens like this requires cell-mediated immunity, which is much harder to measure. It’s also important to remember that even with a vaccine, control of this disease (as with any infectious disease) depends on a lot of other factors, like reducing stress on animals and preventing transmission and spread of the bacteria. Unfortunately, no one is even sure exactly what the source of the Lawsonia is in most foals (although there’s a good chance that they ingest it, possibly with fecal contamination from foals that are shedding Lawsonia).

As more and more cases of Lawsonia are seen, both in North America and now in Europe, researchers will continue to learn more about how this disease is spread, and hopefully one day develop a vaccine to help us prevent it.

Keep watching this site for more of the latest and greatest from the 2008 AAEP Conference!

Equine Viral Diseases

The December 2008 newsletter from the University of Guelph's Animal Health Laboratory contains an article about diagnoses of equine viral infections from 1998-2008. Equine herpesvirus type 1 (EHV-1) was the most commonly detected virus and the most commonly diagnosed infectious cause of abortion. Three cases of neuropathic EHV-1 (the strain that was recently identified as being an important cause of the neurological form of this disease) have been diagnosed since specific testing for this strain started late last year. Small numbers of influenza cases were confirmed and, as expected, they all involved the H3N8 type virus. Eastern equine encephalitis (EEE) is a rare disease in Ontario, but an outbreak occurred in 2008, with 8 cases identified. Equine rhinitis viruses A and B (formerly equine rhinovirus 1 and 2) were found in a few horses with fever, respiratory tract disease and swelling (edema) of the limbs and abdomen.

This newsletter, along with previous versions, can be found here. (Note: at the time of writing, the December newlsetter was not yet available online. If you look and it's not there, check back again later because it should be available soon).

Image: TEM of equine herpesvirus (source: Gluck Equine Research Center)

Guttural Pouch Mycosis: Ticking Time-Bomb

One (of many) unique anatomical aspects of horses is their guttural pouches. These pouches open into the throat area and are essentially large openings in what is the equivalent of a horse's Eustachian tube. They contain several important structures including nerves and major blood vessels such as the carotid artery (see top picture right). The reason horses have guttural pouches is unclear, but it has been proposed that they are used to help cool the blood going to the brain during intense exercise under hot conditions. Regardless of their purpose, horses can develop a variety of problems with their guttural pouches, some of which can be life-threatening.

One such problem is fungal infection of a guttural pouch, which is a condition known as guttural pouch mycosis. This condition may go completely unnoticed for quite some time, but it becomes very serious if the fungus begins to grow over one of the large blood vessels in the pouch, particularly the carotid artery. The fungal infection can weaken the wall of the artery, ultimately causing it to rupture.  This results in massive blood loss (which comes out of the horse's nose) and it is a potentially life-threatening event.

Early signs of guttural pouch mycosis may include a slight bloody nasal discharge, typically from just one nostril. A few other problems can cause this type of discharge too, like an ethmoidal hematoma. However sometimes there are no warning signs before the horse has a major bleed. I've had cases where there was just a trickle of blood reported by the referring veterinarian, but by the time the horse arrived at the hospital, the blood was gushing from the nose like water from a hose. These massive bleeds can lead rapidly to death due to blood loss. Ideally, cases are identified before a major bleed occurs, but this is not always possible. Here are some points to consider:

  • Take all nosebleeds seriously. Don't panic, but have your horse evaluated promptly by your veterinarian in order to identify the source of the blood, even if the cause of the bleeding can't be determined.  The cause of blood coming from a guttural pouch should be considered guttural pouch mycosis until proven otherwise.
  • The fungi that cause guttural pouch mycosis are widespread in the environment. The reason that some horses develop this type of infection while others do not is not known. There is nothing that can be done to prevent it.
  • If guttural pouch mycosis is diagnosed (or suspected), the horse should be referred to an equine hospital. The best way to prevent a severe bleed is to cut off the blood supply to the affected area of the artery, which is done surgically. In the case of the carotid artery, which is connected to several other major blood vessels at the base of the brain by a structure called the Circle of Willis, the blood supply must be blocked both above and below the level of the fungal plaque in the guttural pouch.  If the artery is only blocked on the side closest to the heart, then the collateral circulation from the Circle of Willis can still result in a severe bleed.  This collateral circulation is also the reason that a major artery, like the carotid, can be blocked on one side (right or left) without harming the horse.
  • The fungus in the pouch "feeds" off the blood supply from the affected artery, so when the blood vessel is occluded, the fungal infection becomes much easier to treat.  Treatment of guttural pouch mycosis with anti-fungal medications that are infused into the guttural pouch is typically not effective, and the risk of a fatal bleed is always present, until the blood supply to the affected artery is cut off.

Sporotrichosis: Stop And Smell The Fungus

Sporotrichosis is a fungal infection of the skin and superficial lymph vessels caused by Spororthrix schenckii.  This organism is often found on decaying plant material, although it can also infest living plants like sphagnum moss and rose bushes.  Sporotrichosis is an uncommon condition in horses, but it occurs all over the world. It is most common in tropical and subtropical regions and is rare in northern climates.

Infection can occur when S. schenckii contaminates a damaged area of skin.  The condition progresses very slowly, but after several weeks the fungus may spread from the skin to nearby lymph vessels (lymphatics).  The lesions are most commonly found on a lower limb, but can occur anywhere, depending on where the infection started.  One, several, or many firm round nodules may be found.  Typically they are not itchy or painful to the horse, but as they get bigger the nodules burst and ooze a pus-like discharge.  When the infection tracks up a lymphatic, the nodules may occur in a row along the vessel, appearing like "beads on a necklace".  The lesions may heal and recur numerous times over months or years.  These clinical signs are very suggestive of sporotrichosis, but bacterial lymphangitis should also be considered.  Sometimes the yeast organisms can be seen in the discharge using a microscope, but the definitive diagnosis is made by culturing the fungus.  The treatment of choice is a form of iodine that can be given orally, although more conventional antifungal drugs may also be effective (but also much more expensive).  The prognosis for recovery is good.

Sporotrichosis can also occur in people, and is sometimes called "rose handler's disease".  It is not usually a problem in healthy individuals, but it can be very serious in any person with a weakened immune system.  Horses and other animals are not considered a source of infection for people.  This is because at the body temperature of an animal (37 deg. C), S. schenckii grows in the form of a yeast.  The infectious form, a filamentous fungus, only grows at lower temperatures, and can therefore be found on plants in the environment.  Because it can grow in two different forms, S. schenckii is considered a dimorphic fungus.  Nonetheless, it is still prudent to wear gloves when handling an infected horse.

Helpful vs Harmful: Antibiotic Risks in Horses

The discovery of antibiotics was one of the most important medical advances in history, and these drugs have had an immense impact on human and animal health. While antibiotics have saved countless lives, their use can also be associated with some very serious side effects and complications.  For example, in horses, antibiotic-associated diarrhea (colitis) is a major issue.

By the nature of their intestinal tract, horses at among the highest risk species for developing serious diarrhea associated with antibiotic use.  The intestinal tract of a horse is full of billions of bacteria of different types. These bacteria are important for normal digestion, and also help to prevent infection by "bad" bacteria such as Salmonella and Clostridium difficile.  Antibiotics can disrupt the balance of this complex bacterial population.  The imbalance itself can cause problems, and can also make it easier for bad bacteria to move in, grow and cause disease.

Here are some important points to remember about antibiotics, particularly in horses:

  • Antibiotics can only kill bacteria. They should not be used when a bacterial infection is not present and not likely to occur. Antibiotics are too commonly misused for viral infections in horses, which puts the animals at unnecessary risk.
  • Any antibiotic can cause diarrhea, but some drugs are considered higher risk. Erythromycin and tetracyclines are considered high-risk in most areas of North America. Certain drugs, such as lincomycin and oral penicillins, are such high risk that they should never be used in adult horses.
  • Both oral and injectable antibiotics can also cause diarrhea. Drugs that are injected can still reach the intestinal tract and affect the bacteria there. Some drugs, like tetracycline, are actively pumped into the intestinal tract from the bloodstream, resulting in relatively high concentrations in the intestine, even if the drug was given by injection.
  • While some antibiotics can be purchased over the counter in some areas, antibiotics should never be given without the direct recommendation of a veterinarian.
  • If your horse is being treated with antibiotics and develops diarrhea, contact your veterinarian immediately.
  • There is no known way to reduce the risk of antibiotic-associated diarrhea in horses, other than to avoid unecessary use of antibiotics. Some people treat horses with probiotics or yogurt, but currently there is no evidence that this is beneficial (but it probably doesn't hurt, at least in adult horses).

Another major concern with antibiotic use is the development of antibiotic resistance in bacteria, but that's a topic for another post (or two, or three or more!).

More information about Clostridium difficile and probiotics in horses can be found on the equIDblog Resources page.

Israeli Outbreak Mystery Revealed

Recently, I wrote about a large disease outbreak involving horses in Israel that resulted in a country-wide quarantine. Equine viral arteritis (EVA) has now been identified as the cause of the outbreak. This viral disease can cause a wide range of clinical signs in horses. It is often associated with abortion, but flu-like disease is common in adult horses, and is consistent with what was reported in Israel. The EVA virus is spread mainly by the respiratory route - contact with infectious nasal discharge is a prime means of transmission between horses.

It is currently unclear where the Israeli outbreak originated, or how it was propagated.  Transportation of horses to shows, races and other events with subsequent mixing of horses is a great way to rapidly spread such a virus over a large area. The World Organization for Animal Health (OIE) reports that this virus has never been identified in Israel before. If this is indeed true, it could explain why the outbreak was so rapid and widespread - when a virus encounters a population of horses who don't have any immunity because they have not been exposed or vaccinated, more animals are likely to become sick and spread the virus further.  The massive influenza outbreak in Australia last year was a great example of that.

It will be interesting to see what happens with this outbreak. The implementation of a nationwide quarantine, while certainly disruptive to many people, may have been an important step in limiting further transmission of the virus.  A vaccine for EVA (which cannot be used in pregnant mares) is available, and could be used as part of outbreak control. However, good infection control practices are more important - vaccination will not be effective if it is the only measure taken.

Click here for more information on equine viral arteritis (EVA).

Unknown Infectious Disease Outbreak Hits Israeli Horses

An outbreak of an unknown infectious disease has occurred in Israel, sickening hundreds of horses and possibly causing death in a few. The disease is characterized by fever, lethargy and loss of appetite, yet the cause has not been identified. At least 30 different farms have been affected, and the spread of disease has prompted the Ministry of Agriculture to implement a nation-wide quarantine of horses. All horse events in Israel have been canceled.  Similar precautions were taken when the outbreak of  equine influenza hit Australia last year.

The described clinical signs in affected horses are very non-specific, so it's hard to speculate about the causative pathogen. There are a variety of potential culprits, including equine influenza virus (EIV), but so far testing has not detected EIV or any other known pathogens.  This "mystery" disease does not appear to affect other animals or people (but without knowing what is causing it, this is difficult to say for certain).  It is possible that this is a "new" disease, but the vast majority of infectious disease outbreaks are caused by pathogens that we already know about. Specialists from Britain have been called in to help with the investigation. Hopefully more information will be available soon.

Eastern Equine Encephalitis Kills Emus in Ontario

Eastern equine encephalitis (EEE) was recently identified as the cause of an outbreak of disease in emus on a farm near Brockville, Ontario. EEE is a viral disease that is spread by mosquitoes, and can cause severe neurological disease in horses.  Previous equIDblog posts describe EEE in more detail.

Emus are quite susceptible to EEE.  Although EEE cannot be transmitted fro emus to horses, the relevance of this report to horse owners is that the emu outbreak indicates that the virus is present in mosquitoes in that region.

Most of the emu deaths in this outbreak occurred in mid-October.  It is unclear whether there is any further risk to horses, people or other animal species. Being a mosquito-borne disease, EEE transmission should decrease dramatically after the first few hard frosts in the fall. It is unlikely that there is a significant risk of further EEE transmission in the Brockville area this year.

Vaccination of horses in Ontario at this time of year is probably not useful because of the low risk of mosquito transmission and the time required for immunity to develop after vaccination. However, people should consider the risk of exposure in subsequent years, and try to reduce this risk. This can be done through a combination of avoiding and controlling mosquitoes (which are also important for prevention of West Nile virus infection) and vaccination against EEE. Since EEE is so rare in Ontario, it is not typically considered a "core" vaccine in horses. However, vaccination for EEE should be considered in regions where the disease has previously been identified.

Why Do I Vaccinate My Horse So Often?

A common (and reasonable) question that I get asked periodically is “Why do I vaccinate my horse every year against tetanus but I only get a booster every 10 years?”   There are actually some good reasons for the difference.

Tetanus is a devastating disease caused by the bacterium Clostridium tetani. It used to be very common, but is now rare because of effective vaccination. Although the disease is rare, the bacterium that causes it is very common, and can be found in soil and manure almost anywhere. Horses typically get exposed to C. tetani through soil contamination of  wounds, especially puncture wounds of the foot or lower leg.

  • Horses are probably more at risk of exposure to C. tetani than people, because these types puncture wounds happen frequently in horses, and the wounds are possibly more likely to be contaminated with the tetanus bacterium.
  • Horses are extremely susceptible to tetanus (more than people and most animals), so situations that would not necessarily cause a problem in people could cause tetanus in a horse.

Given their risk of exposure, their susceptibility to the disease, and the difficulty (and high cost) of treating tetanus in horses, and the safety and effectiveness of vaccination, it is recommended that horses are vaccinated frequently for tetanus. It is possible that longer intervals for vaccination than every year could be used, but there is currently no information that tells us how long we can stretch it. So, erring on the side of caution, and considering tetanus vaccination is safe and cheap, it makes sense to vaccinate horses every year.

More information on tetanus is available on the equIDblog Resources page.

Suspected Botulism Outbreak Kills Dozens of Florida Horses

Preliminary evidence has suggested that botulism might be the cause of botulism may be the cause of death of approximately 100 horses at a large breeding facility in Florida housing approximately 400 mares. The horses died over a period of about 7 days, with signs of neurological disease. There are no reports of affected horses at other facilities in the area.  The clinical signs in these horses and lack of evidence of another disease are strongly suggestive of botulism. This farm apparently fed the horses haylage (although some reports seem to alternate between using the words "hay" and "haylage"), which is a high-risk feed source for botulism.

In adult horses, botulism is caused by ingesting a toxin produced by the bacterium Clostridium botulinum. This bacterium will not grow in the presence of oxygen, however in can grow in conditions that are sometimes present in improperly fermented haylage and silage. As the bacterium grows, it produces botulinum toxin, one of the most potent toxins on the planet. Ingestion of botulinum toxin leads to progressive paralysis (i.e. severe weakness and flacid muslces).

Treatment of botulism is difficult and can be expensive, and the mortality rate for this disease is high. Some people choose to feed haylage and silage despite the risk of botulism. If you choose to feed haylage, silage or other high-risk feeds:

  • Ensure that haylage/silage is properly prepared and stored.
  • Consider vaccinating horses that are fed haylage/silage against botulism. Be aware, however, that vaccines do not protect against all strains of botulism.
  • Immediately stop feeding haylage or silage if any horses show signs that could be consistent with botulism (e.g. weakness, problems eating).

Eastern Equine Encephalitis in Ontario

This summer, a horse in Ontario was diagnosed with Eastern Equine Encephalitis (EEE), a serious neurological disease caused by a virus of the same name, which is transmitted by mosquitoes.  The horse was from the North Durham region.  The last reported cases of EEE in Ontario were in 2004.  A previous equIDblog entry talked about a large number of cases of EEE that have been reported in Florida this year.

Here are some of the key points to remember about EEE:

  • Like West Nile, EEE is a seasonal disease.  It is more common in warmer areas, especially some regions of the southeastern US.  It is rare in cooler climates, but occasionally EEE is found in horses in Ontario.
  • EEE is usually fatal in horses, and there is no effective treatment.
  • EEE can also occur in people, and can be fatal in some cases.
  • Infected horses cannot transmit the EEE virus to people, but if a horse gets EEE from the mosquitoes in the area, then people could also potentially be exposed to the virus by mosquitoes.
  • A vaccine for EEE is available for horses, but most horses in Ontario are not vaccinated for EEE because it is so rare.  Nonetheless, vaccination can be considered because the disease is so devastating when it occurs.
  • As for West Nile virus, avoiding mosquitoes - for both horses and people - is an important preventative measure for EEE.

For more information, see the equIDblog post "Eastern Equine Encephalitis – Not Just For Horses", or the CDC's website on arboviral encephalitides.

This equIDblog entry was originally posted on the Worms & Germs blog on 26-Aug-08.

Deadly Hendra Virus Resurfaces in Australia

This summer, a small outbreak of the potentially deadly Hendra virus was identified in a group of horses near Brisbane, Australia. This virus has caused periodic cases of illness and death in horses, and can be transmitted to people working closely with infected horses. In the latest outbreak, 3 horses died, making this the worst outbreak since 1994 when 14 horses and 2 people died.  A human case was also identified. This person worked at a veterinary clinic that treated infected horses. This individual was admitted to hospital overnight but was discharged, so  presumably was not very ill.

While Hendra virus (genus Henipavirus) is only found in Australia, it is a good reminder for everyone about the strange nature of some infectious diseases. The natural reservoir of the virus is the fruit bat. It is believed that horses become exposed when infected fruit bats give birth and contaminate horse pastures with uterine fluids. Horses develop respiratory disease ranging from mild to fatal. Human cases have been reported in people working closely with infected horses. A horse trainer and veterinarian's assistant died in the 1994 outbreak. Close contact is required for transmission to people.

Picture: Locations of previous Henipavirus outbreaks (red stars – Hendra virus; blue stars – Nipah virus) and distribution of Henipavirus flying fox reservoirs (red shading – Hendra virus; blue shading – Nipah virus)

It's very difficult to take specific measures to protect horses, people or other animals from sporadic, rare diseases such as Hendravirus infection. However, common sense infection control measures can reduce the risks associated with any animal contact.

  • Wash your hands after contact with any animal.
  • Avoid contact with sick animals - consider sick animals to be potentially infectious until proven otherwise.
  • Remember that  new animal diseases are regularly being identified, and that they might be able to infect people.
  • People that work in veterinary clinics must be diligent and use good infection control practices because they are at higher risk of exposure to various diseases.

This equIDblog entry was originally posted on the Worms & Germs blog on 15-Jul-08.

Eastern Equine Encephalitis - Not Just For Horses

Over 50 horses have died from Eastern Equine Encephalitis in Florida this year. The disease, caused by a virus of the same name, affects the brain, resulting in a broad range of clinical signs from behaviour changes to blindness to irregular gait. The disease is also sometimes called “sleeping sickness” because some horses may become severely depressed, with low head carriage and droopy eyes, ears and lips. Almost all horses that develop neurological signs from this infection die. Only 35 cases were reported in Florida in 2006 and 2007 combined.

There are actually three related equine encephalitis viruses – Eastern, Western and Venezuelan – which are called EEE, WEE and VEE for short. VEE is found in South and Central America and Mexico, and occasionally in the southern United States, but has never been reported as far north as Canada (VEE is a reportable disease in Canada). It is unique among the three diseases as the only one in which an infected horse will carry enough virus in its bloodstream to infect a mosquito, which could then pass the virus on to another animal. The EEE and WEE viruses, just like the West Nile virus, do not reach high enough levels in the bloodstream of horses to do this. The mosquitoes usually pick up the viruses from passerine birds, which do not become ill from the viruses (unlike West Nile virus in birds from the family Corvidae).

People can also be infected by EEE, WEE and VEE. About 10 fatal cases of EEE in people are reported in the United States every year. But horses cannot transmit EEE or WEE to humans, even if they’re bitten by the same mosquito. A higher number of cases in horses, however, may mean a higher number of mosquitoes that are carrying the virus. There is no vaccine for these viruses for humans, but there are vaccines available for EEE, WEE and VEE for horses.

In the end, EEE is just one more good reason to make sure you wear mosquito repellent when you’re enjoying the great outdoors during the summer. Visit the Health Canada website for safety tips on using personal insect repellents. EEE is very uncommon in Ontario, but horses that live in or travel to the southern United States should be vaccinated. Talk to your veterinarian about whether or not your horse should be vaccinated. Remember that fly control is also important for our equine companions (and also helps protect them against West Nile!).

This equIDblog entry was originally posted on the Worms & Germs blog on 02-Aug-08.

Equine Infectious Disease Information Sheets

Click on any of the highlighted links below for more information about these horse-related infectious disease topics. Topics that are not highlighted are in development and coming soon. New information will be added as it becomes available, so be sure to check this page regularly for the latest updates.

Bacteria Viruses Parasites Other
Clostridium difficile Rabies Bots Pleuropneumonia
Clostridial Myonecrosis Eastern Equine Encephalitis Equine Protozoal Myeloencephalitis (EPM) Neonatal Diarrhea
Strangles (Streptococcus equi) Equine Herpesvirus Cyathostomes
(Small Strongyles)
Needlestick Injuries
MRSA Equine Influenza Large Strongyles Colostrum
Lawsonia West Nile Virus Tapeworms  
Rhodococcus equi   Pinworms  
Tetanus      
Botulism -
Feed-Associated
     
Botulism -
"Shaker Foals"
     
       
       


All information sheets found on this page can be freely downloaded, printed and distributed. The authors only request that this website (www.equIDblog.com) is acknowledged as the source.  The downloadable files on this page can be opened with Adobe® Reader®.  To get the latest version of Adobe® Reader® for free, click here.

Other Infectious Disease Resources